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PerCP/Cyanine5.5 anti-mouse CD11a Antibody

Pricing & Availability
Clone
M17/4 (See other available formats)
Regulatory Status
RUO
Other Names
αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M17slash4_PerCPCy5.5_CD11a_Antibody_FC_120213
C57BL/6 mouse splenocytes were stained with CD11a (clone M17/4) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • M17slash4_PerCPCy5.5_CD11a_Antibody_FC_120213
    C57BL/6 mouse splenocytes were stained with CD11a (clone M17/4) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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101123 25 µg £89
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101124 100 µg £197
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Description

CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/6 mouse splenic secondary cytotoxic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2 and FOXP3 expression5, and immunohistochemical staining of acetone-fixed frozen sections3. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101110). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101118) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
  2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
  3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
  4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
  5. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed
Product Citations
  1. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  2. Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
  3. Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed
  4. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  5. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  6. Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
  7. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  8. Zhao H, et al. 2022. Int J Biol Sci. 18:154. PubMed
RRID
AB_2562932 (BioLegend Cat. No. 101123)
AB_2562932 (BioLegend Cat. No. 101124)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution

Lymphocytes, granulocytes, monocytes, macrophages

Function
Cellular adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type
Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.

Gene ID
16408 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/20/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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