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PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_PerCPCy5.5_CD223_Antibody_FC_082412
Con A-stimulated splenocytes stained with anti-mouse CD223 PerCP/Cyanine5.5 (clone C9B7W) (filled histogram) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • C9B7W_PerCPCy5.5_CD223_Antibody_FC_082412
    Con A-stimulated splenocytes stained with anti-mouse CD223 PerCP/Cyanine5.5 (clone C9B7W) (filled histogram) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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125211 25 µg £61
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125212 100 µg £161
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Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
  2. Balood M, et al. 2022. Nature. 611:405. PubMed
  3. Liu M, et al. 2022. Cells. 11: . PubMed
  4. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  5. Zander R, et al. 2022. Immunity. 55:475. PubMed
  6. Nguyen T, et al. 2023. Nat Commun. 14:3357. PubMed
  7. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  8. Ertl Y 2014. J Immunol. 193:1836. PubMed
  9. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  10. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  11. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  12. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  13. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  14. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  15. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  16. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  17. Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
  18. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  19. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
RRID
AB_2561516 (BioLegend Cat. No. 125211)
AB_2561516 (BioLegend Cat. No. 125212)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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