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PE anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
B8.2C12 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
B8dot2C12_PE_060309.jpg
Mouse CD366 (Tim-3) transfected cells stained with anti-mouse CD366 (Tim-3, clone B8.2C12) PE.
  • B8dot2C12_PE_060309.jpg
    Mouse CD366 (Tim-3) transfected cells stained with anti-mouse CD366 (Tim-3, clone B8.2C12) PE.
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134003 50 µg £92
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134004 200 µg £280
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mTim-3 protein/Freund adjuvant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone B8.2C12 only binds to the BALB/c allele of Tim-3.

Application References

(PubMed link indicates BioLegend citation)
  1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  2. Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed
  3. Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed
  4. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  5. Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed
  6. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  7. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  8. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  9. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  10. Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed
  11. Zhu H, et al. 2018. Elife. 7:. PubMed
  12. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  13. Zhang X, et al. 2020. J Cancer. 11:648. PubMed
  14. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  15. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  16. Puleston D, et al. 2014. Elife. 3:3706. PubMed
RRID
AB_1626177 (BioLegend Cat. No. 134003)
AB_1626177 (BioLegend Cat. No. 134004)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Distribution

Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.

Function
Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
Ligand/Receptor
Galectin -9
Cell Type
Dendritic cells, Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454
3. Mooney L, et al. 2002. Nature. 415:536
4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/10/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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