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Purified anti-BAD Antibody

Pricing & Availability
Clone
W16055A (See other available formats)
Regulatory Status
RUO
Other Names
Bcl2-associated agonist of cell death, Bcl-2-binding component 6 (Bbc6), Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death.
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16055A_PURE_BAD_WB_022317
Total cell lysates (15 µg protein) from HeLa, A431, A20 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 5 µg/mL purified anti-BAD (clone W16055A) antibody. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • W16055A_PURE_BAD_WB_022317
    Total cell lysates (15 µg protein) from HeLa, A431, A20 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 5 µg/mL purified anti-BAD (clone W16055A) antibody. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
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695502 100 µg £189
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Description

BCL-2 associated agonist of cell death (BAD) is a member of the BCL-2 family, which is involved in the regulation of the mitochondrial apoptosis pathway. The BCL-2 protein family members can either promote cell survival (BCL-2, BCL-XL, and MCL-1) or induce apoptosis (BAX, BAK, BIM, BID, and BAD). The BCL-2 family proteins form homodimers or heterodimers through the conserved BCL-2 homology motifs (BH1, BH2, BH3, and BH4). BAD belongs to the BH3-only proteins and functions as a direct antagonist of the prosurvival effect mediated by BCL-2 and BCL-XL. BAD is inactivated by several survival kinases, such as AKT, PKA, and RSK, through phosphorylation. Upon stimulation of survival signals, phosphorylated BAD dissociates from BCL-2/ BCL-XL complexes and is sequestered in the cytoplasm by 14-3-3 scaffold proteins. Without interacting with BAD, BCL-2 and BCL-XL are able to suppress apoptosis by preventing release of cytochrome c from mitochondria and blocking subsequent activation of caspases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length mouse BAD recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chea C, et al. 2018. PLoS One. 13:e0191683. PubMed
RRID
AB_2650854 (BioLegend Cat. No. 695502)

Antigen Details

Structure
204 amino acids with a predicted molecular weight of 22.1 kD. Contains a BH3 domain responsible for dimer formation.
Distribution

Mitochondrion outer membrane and cytoplasm.

Function
BAD is a proapoptotic protein, promoting cell apoptosis through inactivating the BCL-2 family members BCL-2 and BCL-XL.
Interaction
Forms homodimers or heterodimers with other members of the same family. Interacts with S100A10, 14-3-3, AKT1, PIM3, NOL3, and HIF3A.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References

1. Cekanova M, et al. 2015. Exp. Cell. Res. 331:1.
2. Pareja F, et al. 2014. Mol. Cancer Res. 12:987.
3. Sakamaki J, et al. 2011. Proc. Natl. Acad. Sci. USA 108:6085.
4. Yu C, et al. 2004. Mol. Cell. 13:329-40.
5. Cartier A, et al. 2003. Exp. Cell Res. 291:242.
6. Moriishi K, et al. 2002. Virology 292:258.

Gene ID
12015 View all products for this Gene ID
UniProt
View information about BAD on UniProt.org
Go To Top Version: 0    Revision Date: 03/14/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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