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Purified anti-C/EBP β (3 isoforms C/EBP β, LAP, LIP) Antibody

Pricing & Availability
Clone
1H7 (See other available formats)
Regulatory Status
RUO
Other Names
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1H7_Purified_CEBP-B_Antibody_1_032019
Total cell lysates (15 µg total protein) from THP-1 (negative control), Raw264.7, P388D1 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-cEBP β Antibody, clone 1H7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1H7_Purified_CEBP-B_Antibody_1_032019
    Total cell lysates (15 µg total protein) from THP-1 (negative control), Raw264.7, P388D1 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-cEBP β Antibody, clone 1H7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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606202 100 µg £150
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Description

C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination. The 1H7 monoclonal antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence of mouse and rat C/EBP β, LIP, and LAP isoforms. The 1H7 antibody does not recognize human C/EBP β, LIP or LAP.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant LIP
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP, IP, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Recognizes three mouse isoforms: C/EBP ß, LAP, LIP. Does not cross-react with human.

Application References

(PubMed link indicates BioLegend citation)
  1. Su, et al. 2003. J. Biol. Chem. 278:51150. (ChIP)
  2. Blidner AG, et al. 2015. J Immunol. 194:3452. PubMed
Product Citations
  1. Huang JQ, et al. 2021. Front Mol Biosci. 8:725275. PubMed
  2. Ebert SM, et al. 2020. J Biol Chem. 295:2787. PubMed
  3. Blidner A, et al. 2015. J Immunol. 194:3452. PubMed
  4. Sharma N, et al. 2021. Front Mol Biosci. 7:603168. PubMed
RRID
AB_315675 (BioLegend Cat. No. 606202)

Antigen Details

Structure
C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively
Distribution

Nuclear

Function
Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
Interaction
CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
Modification
Phosphorylation, Ubiquitination
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642.
2. Eaton E, et al. 2001. J. Cell Physiol. 189:91.
3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557.
4. Hattori T, et al. 2003. Oncogene. 22:1273.

Regulation
Growth hormone
Gene ID
12608 View all products for this Gene ID 25301 View all products for this Gene ID
UniProt
View information about C/EBP beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01/13/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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