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Purified anti-c-Fos Antibody

Pricing & Availability
Clone
Poly6414 (See other available formats)
Regulatory Status
RUO
Other Names
Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
1_Poly6414_Purified_c-Fos_Antibody_WB_090916
Total cell lysates (15 µg protein) from HT29, HCT116 and Raw264.7 treated with PMA 100ng/mL for one hour or without treatment were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-c-Fos (clone poly6414). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH Antibody was used as a loading control.
  • 1_Poly6414_Purified_c-Fos_Antibody_WB_090916
    Total cell lysates (15 µg protein) from HT29, HCT116 and Raw264.7 treated with PMA 100ng/mL for one hour or without treatment were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-c-Fos (clone poly6414). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH Antibody was used as a loading control.
  • 2_Poly6414_Purified_c-Fos_Antibody_WB_032119
    Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with a 1:1000 dilution of Purified anti-c-Fos Antibody, clone Poly6414, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP anti-rabbit IgG Antibody (Cat. No. 410406) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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641401 25 µL £77
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641402 100 µL £182
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Description

The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 - 1:4000 antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Fan W, et al. 2009. Brain Res Red. 59:324. PubMed
  2. Sugimoto K, et al. 2014. Neurosci Res. PubMed
Product Citations
  1. Cong J, et al. 2022. Theranostics. 12:5204. PubMed
  2. Chung H, et al. 2021. Nat Methods. 18:1204. PubMed
  3. Sugimoto K, et al. 2015. Neurosci Res. 96: 20-29. PubMed
  4. Fan W, et al. 2009. Brain Res Rev. 59:324. PubMed
RRID
AB_2106632 (BioLegend Cat. No. 641401)
AB_2106632 (BioLegend Cat. No. 641402)

Antigen Details

Structure
c-fos heterodimerizes with c-jun to form the transcription factor AP-1; contains a bZIP region with a DNA-binding domain and a leucine zipper domain; approximately 62 kD.
Distribution

Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes.

Function
FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal form
Interaction
Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Modification
Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Dony C, et al. 1987. Nature 328:711.
2. Grigoriadis AE, et al. 1994. Science 266:443.
3. Glover JNM, et al. 1995. Nature 373:257.

Regulation
c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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