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Purified anti-CD142 Antibody

Pricing & Availability
Clone
A22071J (See other available formats)
Regulatory Status
RUO
Other Names
Coagulation Factor III, Tissue Factor, TF, Thromboplastin, platelet tissue factor, factor III, factor 3, F3, F-3, TFA, P13726
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A22071J_PURE_CD142_WB_081924
Whole cell extracts (15 µg total protein per lane) from indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CD142 (clone A22071J) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A22071J_PURE_CD142_WB_081924
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CD142 (clone A22071J) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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681451 25 µg £117
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681452 100 µg £281
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Description

CD142, also known as Tissue Factor (TF), Coagulation Factor III, and Thromboplastin, is a 45 kD type I transmembrane glycoprotein. It is expressed on the surface of a variety of cells that are physically separated from the circulating blood which include smooth muscle cells, fibroblasts, keratinocytes, glomerular epithelial cells (cytoplasmic inclusions), astrocytes, myocardium, liver stromal cells, pancreas cells, and is also expressed on activated monocytes and stimulated endothelial cells. CD142 is a high-affinity receptor for coagulation factor VII/VIIa and initiates the extrinsic pathway of blood coagulation. CD142 also plays an important role in a variety of diseases such as sepsis, atherosclerosis, and cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human CD142
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone A22071J) is not recommended for immunocytochemistry (ICC) or Immunohistochemistry in formalin-fixed paraffin-embedded tissue (IHC-P).

RRID
AB_3662336 (BioLegend Cat. No. 681451)
AB_3662336 (BioLegend Cat. No. 681452)

Antigen Details

Structure
45 kD Type I transmembrane glycoprotein
Distribution

Smooth muscle cells, fibroblasts, keratinocytes, glomerular epithelial cells, astrocytes, myocardium, liver stromal cells, pancreas cell, stimulated endothelial cells, stimulated monocytes

Function
Blood coagulation
Ligand/Receptor
Coagulation Factor VIIa
Cell Type
Astrocytes, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Carson SD, et al. 1994. Blood. 84:526-34.
  2. McComb RD, et al. 1991. Am J Pathol. 139:491-4.
  3. Whittle SM, et al. 1995. Thromb Res. 79:451-9.
Gene ID
2152 View all products for this Gene ID
UniProt
View information about CD142 on UniProt.org
Go To Top Version: 1    Revision Date: 08/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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