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Purified anti-CD31 (PECAM-1) Antibody

Pricing & Availability
Clone
JC70 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet and endothelial cell adhesion molecule (PECAM-1), EndoCAM
Isotype
Mouse IgG1, κ
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Product Citations
publications
a. JC70_PURE_CD31_WB_Antibody_092223
Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-CD31 (PECAM-1) (clone JC70) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. JC70_PURE_CD31_WB_Antibody_092223
    Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-CD31 (PECAM-1) (clone JC70) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. JC70_PURE_CD31_IHC-P_Antibody_091123
    IHC staining of purified anti-CD31 (PECAM-1) (clone JC70) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective. Scale bar: 50 µm
  • c. JC70_PURE_CD31_IHCP_Antibody_091123
    IHC staining of purified anti-CD31 (PECAM-1) (clone JC70) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated with 5 µg/mL of purified anti-CD31 (PECAM-1) (clone JC70) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • d. JC70_PURE_CD31_ICC_Antibody_091123
    A431 cells (negative cell line) (panel A) and HEL cells (positive cell line) (panel B) were fixed with 4% PFA and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5.0 µg/mL purified anti-CD31 (PECAM-1) (clone JC70), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) and the image was captured with a 60X objective. Scale: 50 µm
  • E. JC70_PURE_CD31_FC_Antibody_091123
    HEL cells (positive control) (filled histogram) and A431 cells (negative control) (open histogram) were intracellularly stained with purified anti-CD31 (PECAM-1) (clone JC70) or purified Mouse IgG1, κ isotype control (Cat. No. 400102) (open histogram, dashed line) followed by PE Goat anti-mouse IgG (Cat. No. 405307).
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623551 25 µg £117
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623552 100 µg £291
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Description

CD31, widely known as “platelet and endothelial cell adhesion molecule” (PECAM), is a crucial transmembrane glycoprotein primarily found on the surface of endothelial cells and platelets. The quintessential endothelial cell marker, CD31 is vital for the maintenance of vascular integrity. It plays a pivotal role in cell-cell adhesion, promoting interactions between neighboring endothelial cells, and facilitates leukocyte diapedesis during the inflammatory response. CD31 also mediates platelet-endothelial interactions, contributing to platelet aggregation and thrombus formation at sites of vascular injury. Additionally, this multifunctional molecule is involved in signal transduction pathways that govern cell migration, proliferation, and survival, making it a key player in immune response regulation and angiogenesis. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cell membrane preparation from the spleen of a patient with hairy cell leukemia
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

JC70 does not cross-react with mouse CD31 in Western Blot.

For ICC we recommend fixation/permeabilization with 100% ice-cold methanol, or 4% PFA followed by 100% ice-cold methanol. Fixation/permeabilization with 4% PFA followed by 0.5% Triton-X results in a significantly dimmer signal.

For IHC-P, we recommend antigen retrieval using Sodium Citrate, pH 6.0 (Cat. No. 420901). Product does not work with Tris-EDTA antigen retrieval.

RRID
AB_3083412 (BioLegend Cat. No. 623551)
AB_3083412 (BioLegend Cat. No. 623552)

Antigen Details

Structure
CD31 is a 738 amino acid protein with a predicted molecular weight of 82.5 kD.
Distribution

Endothelial cells, Platelets, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Cell surface

Function
Cell adhesion
Interaction
BDKRB2 and GNAQ
Ligand/Receptor
Forms homophillic interactions, also CD38, CD177, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. DeLisser HM, et al. 1994. Immunol. Today. 15:490-5.
  3. Newman PJ, et al. 1990. Science 247:1219-22.
  4. Lertkiatmongkol P, et al. 2016. Curr Opin Hematol. 23:253-9.
  5. Yeh JC, et al. 2008. Biochemistry. 47:9029-39.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 1    Revision Date: 09/11/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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