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Purified anti-CD33 Antibody (Previously Covance catalog# MMS-5090)

Pricing & Availability
Clone
P67.6 (See other available formats)
Regulatory Status
RUO
Other Names
Myeloid cell surface antigen CD33, CD33 antigen (gp67), sialic acid binding Ig-like lectin 3,gp67, SIGLEC3, Siglec3
Previously
Covance Catalog# MMS-5090
Isotype
Mouse IgG1, κ
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Product Citations
publications
P67.6_Purified_CD33_Antibody_IHC_032515
IHC staining of purified anti-CD33 antibody (clone P67.6) on frozen human spleen tissue. The tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • P67.6_Purified_CD33_Antibody_IHC_032515
    IHC staining of purified anti-CD33 antibody (clone P67.6) on frozen human spleen tissue. The tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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825601 100 µL £189
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against FMY9S5 cells containing the CD33 gene.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-F - Quality tested
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 10.0 - 20.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemical staining on frozen tissue (IHC-F) and flow cytometry (FC).

Application References

(PubMed link indicates BioLegend citation)
  1. Favaloro EJ, et al. 1987. Dis. Markers. 5:215. [clone characterization]
  2. Andrews RG, et al. 1983. Blood. 62:124. (FC)
  3. van der Jagt RH, et al. 1992. Cancer Res. 52:89. (FC)
  4. Liesz A, et al. 2015. J. Neurosci. 35:583. (FC)
Product Citations
  1. Dao T, et al. 2022. JCI Insight. 7: . PubMed
  2. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  3. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  4. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  5. Shaw BC, et al. 2021. CRISPR J. 4:699. PubMed
RRID
AB_2564890 (BioLegend Cat. No. 825601)

Antigen Details

Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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