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Purified anti-human CD33 Antibody

Pricing & Availability
Clone
P67.6 (See other available formats)
Regulatory Status
RUO
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P67.6_Purified_CD33_Antibody_FC_032315
Human peripheral blood monocytes were stained with purified CD33 (clone P67.6, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG FITC.
  • P67.6_Purified_CD33_Antibody_FC_032315
    Human peripheral blood monocytes were stained with purified CD33 (clone P67.6, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG FITC.
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366601 25 µg £37
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366602 100 µg £85
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Description

CD33, also known as Siglec-3, gp67, and p67, is a 67 kD type I transmembrane glycoprotein. It is a sialoadhesion immunoglobulin superfamily member, which is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells, and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FMY9S5 cells expressing CD33 gene.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hoyer J, et al. 2008. Am. J. Clin. Pathol. 129:316.
Product Citations
  1. Wißfeld J, et al. 2021. Sci Rep. 11:13462. PubMed
  2. Schapansky J, et al. 2020. J Biol Chem. 296:100218. PubMed
  3. NULL, et al. 2022. Cell. 185:916. PubMed
RRID
AB_2565538 (BioLegend Cat. No. 366601)
AB_2565538 (BioLegend Cat. No. 366602)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, and mast cells.

Function
Adhesion and lectin binding activity.
Ligand/Receptor
Sugar chains containing sialic acid.
Cell Type
Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org
Go To Top Version: 1    Revision Date: 03/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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