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Purified anti-CD49C (Integrin α3) Antibody

Pricing & Availability
Clone
P1B5 (See other available formats)
Regulatory Status
RUO
Other Names
GAP-B3, GAPB3, ILNEB, MSK18, VCA-2, VL3A, VLA3a, Integrin alpha-3, FRP-2, galactoprotein B3, VLA-3 subunit alpha, CD49 antigen-like family member C, antigen identified by monoclonal antibody J143, alpha-3 subunit
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1B5_Purified_CD49C_Antibody_1_041719.png
IHC staining of purified anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Purified_CD49C_Antibody_1_041719.png
    IHC staining of purified anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Purified_CD49C_Antibody_2_041719.png
    ICC staining of purified anti-CD49C (Integrin α3) antibody (clone P1B5) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Purified_CD49C_Antibody_3_041719.png
    HeLa cells were fixed with 4% PFA, permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002), and stained with purified anti-CD49C (Integrin α3) antibody (clone P1B5, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE(Cat. No. 405307).
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865201 25 µg £73
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865202 100 µg £189
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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1B5 monoclonal antibody was generated against human HT1080 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
FC, ICC - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is between 1.0 - 10 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes the a3 subunit.

Application References

(PubMed link indicates BioLegend citation)
  1. Wayner EA and Hoffstrom BG. 2007. Methods. Enzymol. 426:117.
  2. Wayner EA, et al. 1993. J Cell Biol. 121:1141. (IP)
  3. Carter WG, et al. 1990. J Cell Biol. 110:1387-404 (IP)
  4. Wayner EA and Carter WG. 1987. J Cell Biol. 105:1873. (IP)
RRID
AB_2810769 (BioLegend Cat. No. 865201)
AB_2810769 (BioLegend Cat. No. 865202)

Antigen Details

Structure
CD49c is a 150 kD type I transmembrane glycoprotein.
Distribution

Cellular distribution: Plasma membrane.
Tissue distribution: Endothelial cells, epithelial cells, and dermal fibroblast.

Function
CD49c plays a role in cell-cell and cell-matrix adhesion.
Ligand/Receptor
Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
3675 View all products for this Gene ID
UniProt
View information about CD49c on UniProt.org
Go To Top Version: 1    Revision Date: 04/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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