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Purified anti-Claudin-1 Antibody

Pricing & Availability
Clone
9E12C72 (See other available formats)
Regulatory Status
RUO
Other Names
Senescence-associated epithelial membrane protein, CLDN1, CLD1, SEMP1,
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E12C72_PURE_Claudin-1_Antibody_051017
Total cell lysates (15 µg protein) from 293T (negative control), A431 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Claudin-1 (clone 9E12C72) antibody, competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Claudin-1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: molecular weight ladder and M* indicates extended exposure of the molecular weight ladder.
  • 9E12C72_PURE_Claudin-1_Antibody_051017
    Total cell lysates (15 µg protein) from 293T (negative control), A431 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Claudin-1 (clone 9E12C72) antibody, competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Claudin-1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: molecular weight ladder and M* indicates extended exposure of the molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
697801 25 µg £70
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697802 100 µg £174
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Description

Claudin-1 is a member of the claudin family of proteins, which is composed of at least 24 members. Claudin proteins are integral membrane proteins with 4 transmembrane regions and 2 extracellular loops that form tight junctions. Tight junctions function as a channel and a barrier to maintain cellular morphology, polarity, and homeostasis between epithelial cells. Claudin-1 is a main component of tight junctions and is sorely responsible for normal barrier function of the skin. Misregulated expression of Claudin-1 has been shown to be associated with cancer progression and metastasis in several cancers. Claudin-1 has been found to be overexpressed in neoplasia associated with inflammatory bowel disease. Mutations in Claudin-1 gene lead to human skin diseases and Claudin-1 deficient mice die soon after birth due to dehydration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic Claudin-1 peptide conjugated to KLH (TPRPYPKPAPSSGKDYVC)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has no cross-reactivity with mouse (in house tested).

RRID
AB_2687191 (BioLegend Cat. No. 697801)
AB_2687191 (BioLegend Cat. No. 697802)

Antigen Details

Structure
211 amino acids with a predicted molecular weight of 22.7 kD and observed approximately 18kD. Multi-pass membrane protein with 4 transmembrane domains.
Distribution

Cell membrane. Cell junction.

Function
Claudin-1 is an integral membrane protein that forms tight junctions between skin epithelial cells to form barriers, regulate paracellular transport, and maintain cell polarity.
Interaction
Forms homopolymers and heteropolymers with other claudin family proteins. Interacts with CLDN3, TJP1/ZO-1, TJP2/ZO-2, TJP3/ZO-3, MPDZ, and PATJ.
Cell Type
Mesenchymal Stem Cells
Biology Area
Stem Cells
Antigen References

1. Suh Y, et al. 2017. Oncogene. 36:1167.
2. Tokumasu R, et al. 2016. Proc. Natl. Acad. Sci. USA. 113:E4061.
3. Zhou B, et al. 2015. J. Clin. Med. 4:1960.
4. Saeedi BJ, et al. 2015. Mol. Biol. Cell. 26:2252.
5. Huang J, et al. 2015. Oncotarget. 6:1652.
6. Izraely S, et al. 2015. Int. J. Cancer. 136:1296.

Gene ID
9076 View all products for this Gene ID
UniProt
View information about Claudin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/12/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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