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- Clone
- 6C9/C3b (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Complement Component 3, C3b, iC3b, C3bi
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot of clone 6C9/3Cb. Lane 1: Molecular weight marker; Lane 2: Human C3 recombinant protein; Lane 3: human C3b recombinant protein; Lane 4: Human plasma. The blot was incubated with clone 6C9/C3b at 10 µg/ml for 24 hours at 4°C, followed by detection using an HRP Goat anti-mouse IgG Antibody. -
Human peripheral mononuclear cells were incubated with purified anti-human CD3 antibody then incubated with human serum. The cells were then stained with purified anti-human C3/C3b/iC3b (clone 6C9/C3b) antibody (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG1 PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 846502 | 100 µg | £182 | ||||
Complement component 3 (C3) is a 185 kD glycoprotein composed of two chains linked by a disulfide bond. It is the fourth complement component to react in the classical pathway and is a key protein in the alternative and the lectin complement activation pathways. C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35). Opsonization of target surfaces with C3b is central to all three pathways of complement activation. The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H. A ~2 kD fragment of C3b, C3f, is generated when C3b is cleaved at two positions by factor I. Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b/CD18) can expand target-specific B and T cells. iC3b, can be cleaved to form C3c and C3dg. Further proteolytic cleavage of C3dg generates C3d and C3g.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human C3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
FC, Direct ELISA - Verified
ELISA Detection - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 µg per million cells in 100 µl volume. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody recognizes human C3, C3b and iC3b, and does not cross-react with C3d.
Personal communication from the developer of clone 6C9/C3b indicates it recognizes mouse and non-human primate C3, C3b, and iC3b.
-
Application References
(PubMed link indicates BioLegend citation) -
- Shi J, et al. 2014. Blood. 123:4015. (FC)
- RRID
-
AB_2572183 (BioLegend Cat. No. 846502)
Antigen Details
- Structure
- C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.
- Distribution
-
Extracellular, plasma membrane, and serum.
- Function
- C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
- Interaction
- Immune cells interact with C3b and iC3b using CR1 and CR2/CR3, respectively.
- Ligand/Receptor
- C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b/CD18).
- Biology Area
- Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Antigen References
-
1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.
- Gene ID
- 718 View all products for this Gene ID
- UniProt
- View information about C3/C3b/iC3b on UniProt.org
Other Formats
View All C3/C3b/iC3b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-complement C3/C3b/iC3b | 6C9/C3b | WB,FC,Direct ELISA,ELISA Detection |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-complement C3/C3b/iC3b
Western blot of clone 6C9/3Cb. Lane 1: Molecular weight mark... 
Human peripheral mononuclear cells were incubated with purif...
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