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Purified anti-complement C3/C3b/iC3b Antibody

Pricing & Availability
Clone
7C12/C3b (See other available formats)
Regulatory Status
RUO
Other Names
Complement Component 3, C3b, iC3b, C3bi
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7C12slashC3b_PURE_complement_Antibody_1_WB_031016
Western blot of clone 7C12/C3b. Lane 1: Molecular weight marker; Lane 2: 2 µg C3 recombinant protein; Lane 3: 2 µg C3b recombinant protein; Lane 4 and 5: Human plasma; Lane 6: BSA negative control. The blot was incubated with clone 7C12/C3b at 10 µg/ml for 24 hours at 4°C. Following primary antibody, the blot was incubated with fluorescent labeled goat anti-mouse secondary antibody.
  • 7C12slashC3b_PURE_complement_Antibody_1_WB_031016
    Western blot of clone 7C12/C3b. Lane 1: Molecular weight marker; Lane 2: 2 µg C3 recombinant protein; Lane 3: 2 µg C3b recombinant protein; Lane 4 and 5: Human plasma; Lane 6: BSA negative control. The blot was incubated with clone 7C12/C3b at 10 µg/ml for 24 hours at 4°C. Following primary antibody, the blot was incubated with fluorescent labeled goat anti-mouse secondary antibody.
  • 7C12slashC3b_PURE_complement_Antibody_2_FC_031016
    Human peripheral mononuclear cells were incubated with purified anti-human CD3 antibody then incubated with human serum. The cells were then stained with purified anti-human C3/C3b/iC3b (clone 7C12/C3b) antibody (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG1 PE.
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846402 100 µg £182
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Description

Complement component 3 (C3) is a 185 kD glycoprotein composed of two chains linked by a disulfide bond. It is the fourth complement component to react in the classical pathway and is a key protein in the alternative and the lectin complement activation pathways. C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35). Opsonization of target surfaces with C3b is central to all three pathways of complement activation. The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H. A ~2 kD fragment of C3b, C3f, is generated when C3b is cleaved at two positions by factor I. Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b/CD18) can expand target-specific B and T cells. iC3b can be cleaved to form C3c and C3dg. Further proteolytic cleavage of C3dg generates C3d and C3g.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human iC3b coupled to sepharose 4B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
FC, Direct ELISA - Verified
ELISA Detection, RIA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 µg per million cells in 100 µl volume. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA Detection1 and RIA2.

This antibody recognizes human C3, C3b and iC3b, and does not cross-react with C3d.

Application References

(PubMed link indicates BioLegend citation)
  1. Tosic L, et al. 1989. J. Immunol. Methods 120:241. (ELISA)
  2. Sokoloff MH, et al. 2000. Cancer Immunol. Immunother. 49:551. (RIA, FC)
  3. Kennedy AD, et al. 2003. Blood 101:1071. (FC)
  4. Lindorfer MA, et al. 2010. Blood 115:2283. (FC)
  5. Beurskens, et al. 2012. J. Immunol. 188:3532. (FC)
Product Citations
  1. Rose N, et al. 2022. Acta Neuropathol. 144:1005. PubMed
RRID
AB_2572175 (BioLegend Cat. No. 846402)

Antigen Details

Structure
C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.
Distribution

Extracellular, plasma membrane, and serum.

Function
C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
Interaction
Immune cells interact with C3b and iC3b using CR1 and CR2/CR3, respectively.
Ligand/Receptor
C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b/CD18).
Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.

Gene ID
718 View all products for this Gene ID
UniProt
View information about C3/C3b/iC3b on UniProt.org

Other Formats

View All C3/C3b/iC3b Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-complement C3/C3b/iC3b 7C12/C3b WB,FC,Direct ELISA,ELISA Detection,RIA
Go To Top Version: 1    Revision Date: 03/11/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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