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Purified anti-Cre Recombinase Antibody (Previously Covance catalog# PRB-106P)

Pricing & Availability
Clone
Poly9080 (See other available formats)
Regulatory Status
RUO
Other Names
Cyclization recombinase
Previously
Covance Catalog# PRB-106P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly9080_Pure_Cre-Recombinase_Antibody_1_091420
Total cell lysates (15 µg total protein) from untransfected (-) or Cre recombinase-transfected (+) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:10000 dilution) of purified anti-Cre recombinase antibody (Poly9080) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker.
  • Poly9080_Pure_Cre-Recombinase_Antibody_1_091420
    Total cell lysates (15 µg total protein) from untransfected (-) or Cre recombinase-transfected (+) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:10000 dilution) of purified anti-Cre recombinase antibody (Poly9080) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker.
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908002 25 µL £61
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908001 200 µL £245
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Description

Cre-recombinase is a 38 kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. This polyclonal antibody was raised against a full-length Cre Recombinase recombinant protein and was subsequently purified using Protein A. It can be used to determine the success of gene manipulations in transgenic animals.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
P1 Bacteriophage
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution + 1% BSA and 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.025 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Zwiers PJ, et al. 2022. PLoS One. 17:e0268986. PubMed
  2. Scekic-Zahirovic J, et al. 2021. Prog Neurobiol. 200:101972. PubMed
  3. Vantrease JE, et al. 2022. J Neurosci. . PubMed
  4. Dvorakova M, et al. 2016. Sci Rep. 6:38253. PubMed
  5. Bäck S, et al. 2019. Neuron. 102:105. PubMed
  6. Anderson T, et al. 2016. Nature. 10.1038/nature18944. PubMed
  7. Karen J Tonsfeldt et al. 2019. Journal of the Endocrine Society. 3(4):716-733 . PubMed
  8. Bohuslavova R, et al. 2021. Int J Mol Sci. 22:. PubMed
  9. Holt LJ, et al. 2018. Endocrinology. 159:1339. PubMed
  10. Twining R, et al. 2017. Nat Neurosci. 20:459-469. PubMed
  11. Dvorakova M, et al. 2020. Dev Biol. 457:43:00. PubMed
  12. Leiton CV, et al. 2018. Hypoxia (Auckl). 6:35. PubMed
  13. Filova I, et al. 2020. Mol Neurobiol. 57:5307. PubMed
  14. Choe Y, et al. 2018. Brain Plast. 4:169. PubMed
  15. Jecrois ES, et al. 2021. Developmental Cell. :. PubMed
  16. Costa TDF, et al. 2019. Nat Commun. 10:3589. PubMed
  17. Dudok J, et al. 2016. Neurosci Res. S0168-0102(16)00002-X. PubMed
  18. Chung EJ, et al. 2021. Int J Radiat Oncol Biol Phys. 110:526. PubMed
  19. Heuer A, et al. 2016. Exp Neurol. 282:78-85. PubMed
  20. Benedykcinska A, et al. 2016. Dis Model Mech. 9: 211 - 220. PubMed
  21. Hoffmann HM, et al. 2019. Neuroendocrinology. 108:328. PubMed
RRID
AB_2565079 (BioLegend Cat. No. 908002)
AB_2565079 (BioLegend Cat. No. 908001)

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Go To Top Version: 6    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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