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Purified anti-E. coli CRP Antibody

Pricing & Availability
Clone
1D8D9 (See other available formats)
Regulatory Status
RUO
Other Names
cAMP receptor protein, cAMP regulatory protein, Catabolite gene activator, cap, csm
Isotype
Mouse IgG1
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Product Citations
publications
1D8D9_Pureaf_EcoliCRP_Antibody_WB_030515
Western blot analysis of 50 ng recombinant CRP protein (lane 1), E. coli (BL21) (lane 2), E. coli (DH5α) (lane 3), and E. coli (Rosetta) (lane 4). Anti- E. coli CRP antibody (clone 1D8D9 ) was used, followed by HRP anti-mouse IgG antibody.
  • 1D8D9_Pureaf_EcoliCRP_Antibody_WB_030515
    Western blot analysis of 50 ng recombinant CRP protein (lane 1), E. coli (BL21) (lane 2), E. coli (DH5α) (lane 3), and E. coli (Rosetta) (lane 4). Anti- E. coli CRP antibody (clone 1D8D9 ) was used, followed by HRP anti-mouse IgG antibody.
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664304 100 µg £221
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Description

CRP (also known as cAMP receptor protein) is a regulatory protein in bacteria. CRP binding to cAMP leads to conformational changes in CRP resulting in a dramatic increase in its binding to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA and acts as a negative regulator of its own synthesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified protein
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Huang Y, et al. 2022. Gut Microbes. 14:2136460. PubMed
  2. Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed
  3. Lauritsen I, et al. 2021. Nat Commun. 12:5880. PubMed
  4. Ayhan D, et al. 2016. PLoS Biol. 14: 1002552. PubMed
  5. Seok JY, et al. 2021. Cell Reports. 36(8):109589. PubMed
  6. Pryor R et al. 2019. Cell. 178(6):1299-1312 . PubMed
RRID
AB_2565553 (BioLegend Cat. No. 664304)

Antigen Details

Structure
210 amino acids with a predicted molecular weight of approximately 23.5 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Ligand/Receptor
cAMP
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Busby S, et al. 1999. J. Mol. Biol. 293:199.
2. Lawson CL, et al. 2004. Curr. Opin. Struct. Biol. 14:10.
3. Hudson BP, et al. 2009. Proc. Natl. Acad. Sci. USA 106:19830.

Gene ID
1036667 View all products for this Gene ID
UniProt
View information about CRP on UniProt.org

Related FAQs

There are no FAQs for this product.

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Go To Top Version: 2    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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