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Purified anti-FGF-1-acidic Antibody

Pricing & Availability
Clone
11A5C44 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast growth factor 1 (FGF-1), Acidic fibroblast growth factor (aFGF), FGF-acidic, Endothelial cell growth factor (ECGF), Heparin-binding growth factor 1 (HBGF-1), FGF1, HBGF1
Isotype
Mouse IgG2b, λ
Ave. Rating
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Product Citations
publications
11A5C44_PURE_FGF-1_Antibody_WB_062215
5, 10, 20, or 50 ng of human FGF-1 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-1-acidic (clone 11A5C44) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 11A5C44_PURE_FGF-1_Antibody_WB_062215
    5, 10, 20, or 50 ng of human FGF-1 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-1-acidic (clone 11A5C44) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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678602 100 µg £157
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Description

FGF-1, one of the most studied members of the fibroblast growth factor family, is a powerful mitogen exhibiting strong action on many different cell types. FGF-1 activity can be mediated not only by autocrine/paracrine pathways, but also by an intracrine pathway. FGF-1 lacks a secretion signal peptide and is exported through a non-classical pathway. Endogenous FGF-1 is found in the nucleus of most cell types. Nuclear localization is required for FGF-1 mitogenic activity. FGF-1 promotes tumor development by promoting cancer cell proliferation and survival. Increased FGF-1 expression in early stages of many different cancers have been reported. MCF-7 breast cancer cell lines that overexpress FGF-1 can form vascularized, metastatic tumors when injected into ovariectomized or tamoxifen-treated nude mice. FGF-1 also induces angiogenesis in vitro and in vivo. Thus, FGF-1 is an attractive candidate for cancer immunotargeting. FGF-1 is also involved in neuronal cell differentiation and survival. FGF-1 is highly expressed in motor neurons. In response to damage, motor neurons can release FGF-1, which results in astrocyte activation. Under oxidative stress, astrocytes can also release FGF-1, which stimulates ApoE/HDL generation in an autocrine manner for protection of the brain against oxidative stress. The involvement of FGF-1 in inflammation, cardioprotection, wound healing, adipocyte remodeling, and restenosis have also been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Parital human human FGF-1 recombinant protein (16-155 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565906 (BioLegend Cat. No. 678602)

Antigen Details

Structure
The 140 amino acid recombinant protein has a predicted molecular mass of approximately 16 kD. Growth factor.
Distribution
Secreted. FGF-1 is widely expressed in developing and adult tissues.
Function
FGF-1 plays an important role in many biological processes, including cell proliferation, inflammation, and angiogenesis. FGF-1 has a low stability and a very short half-life in vivo. Binding to heparin increases the stability of FGF-1 and is also important for the formation of active FGF-acidic/FGFR complex.
Interaction
Intracellular FGF-1 can interact directly with CK2, FIBP, mortalin, and the ribosome-binding protein p34/leucine-rich repeat containing 59 (LRRC59).
Ligand/Receptor
FGF-1 can signal through all known cell-surface FGFR isoforms (FGFR1b, 1c, 2b, 2c, 3b, 3c, and 4). FGF-1 also binds to heparin and heparin sulfate proteoglycans on the cell surface.
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Growth Factors, Cytokines/Chemokines
Antigen References

1. Presta M, et al. 2005. Cytokine Growth Factor Rev. 2:159.
2. Zakrzewska M, et al. 2008. Crit. Rev. Clin. Lab. Sci. 45:91.
3. Zhang L, et al. 1997. Oncogene. 15:2093.
4. Lin YZ, et al. 1996. J. Biol. Chem. 271:5305.
5. Culajay JF, et al. 2000. Biochemistry 39:7153.
6. Cassina P, et al. 2005. J. Neurochem. 93:38.
7. Pehar M, et al. 2005. Neurodegener. Dis. 2:139.
8. Jonker JW, et al. 2012. Nature 485:391.
9. Compagni A, et al. 2000. Cancer Res. 60:7163.

Gene ID
2246 View all products for this Gene ID
UniProt
View information about FGF-1-acidic on UniProt.org

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Go To Top Version: 2    Revision Date: 09/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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