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Purified anti-Filaggrin Antibody

Pricing & Availability
Clone
Poly19058 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal filaggrin
Isotype
Rabbit Polyclonal
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Product Citations
publications
Poly19058_PURE_Filaggrin_Antibody_IHCF_062216
IHC staining of anti-Filaggrin antibody (Poly19058) on frozen human skin tissue. The tissue was incubated with the primary antibody at 2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • Poly19058_PURE_Filaggrin_Antibody_IHCF_062216
    IHC staining of anti-Filaggrin antibody (Poly19058) on frozen human skin tissue. The tissue was incubated with the primary antibody at 2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
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905804 100 µg £245
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Description

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Non-Human Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide corresponding to residues 24-39 in the partial sequence of filaggrin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wallace L, et al. 2012. J. Cell Sci. 125:1750.
  2. Bayo P, et al. 2008. Endocrinology 149:1377.
  3. Hu Y, et al. 2001. Nature 410:710.
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
  5. Liang Y. 2011. Patholog. Res. Int. 2011:936794. (IHC) PubMed
  6. Roop DR, et al. 1984. J. Biol. Chem. 259:8037.
Product Citations
  1. Miyauchi K, et al. 2021. Front Immunol. 12:663177. PubMed
  2. Baur R, et al. 2021. Toxicol Sci. 184:223. PubMed
  3. Hwang JH, et al. 2021. Int J Mol Sci. 22:00. PubMed
  4. Noguchi S, et al. 2019. Commun Biol. 2:37. PubMed
  5. Lee W, et al. 2019. Nutrients. 11. PubMed
  6. Kim SK, et al. 2022. Allergy Asthma Immunol Res. 14:549. PubMed
  7. Kim BE, et al. 2021. JCI Insight. 125:1677. PubMed
  8. Zheng H, et al. 2022. Cell Death Dis. 13:635. PubMed
RRID
AB_2616957 (BioLegend Cat. No. 905804)

Antigen Details

Structure
Filaggrin is a 44 kD protein.
Distribution

Cytoskeleton and nucleus.

Function
Mutations in this gene are associated with ichthyosis vulgaris.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Gene ID
2312 View all products for this Gene ID
UniProt
View information about Filaggrin on UniProt.org
Go To Top Version: 3    Revision Date: 08/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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