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Purified anti-GD3 (MEL-1) Antibody (Previously Covance catalog# SIG-38110)

Pricing & Availability
Clone
R24 (See other available formats)
Regulatory Status
RUO
Other Names
GD3S, SIAT8, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, SIAT8-A, ganglioside GD3 synthase, sialytransferase St8Sia I, alpha-2,8-sialyltransferase 8A, disialoganglioside (GD3) synthase, ganglioside-specific alpha-2,8-polysialyltransferase
Previously
Signet Catalog# 8110-01
Covance Catalog# SIG-38110
Isotype
Mouse IgG3, κ
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Product Citations
publications
R24_Purified_MEL-1_Antibody_IHC_032615
Staining of MEL-1 (R24) on frozen human melanoma tissue.
  • R24_Purified_MEL-1_Antibody_IHC_032615
    Staining of MEL-1 (R24) on frozen human melanoma tissue.
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917701 1 mL £269
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Description

GD3, also known as MEL-1, is a ganglioside found in almost all melanomas and is an important biomarker for malignant melanoma. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. Anti-GD3 antibodies have been shown to induce partial remission of tumor growth due to the proliferation of cytotoxic lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against SK-MEL-28 melanoma cell line.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 with for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: The preferred specimens for this antibody are frozen tissues and/or cytocentrifuge preparations fixed 10 minutes in 4°C acetone.
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Positive tissue (human): Melanoma

MEL-1 (clone R24) recognizes disialoganglioside GD3, a prominent cell surface marker of human melanoma cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Meidenbauer N, et al. 2004. Cancer Res. 64: 6319.
  2. Kasahara K, et al. 2000. J. Biol. Chem. 275: 34701.
  3. Watarai S, et al. 1991. J. Biochem. 110: 889.
  4. Yuyama K, et al. 2007. J. Biol. Chem. 282: 26392.
Product Citations
  1. Buchholz DE, et al. 2020. Proc Natl Acad Sci U S A. 117:15085. PubMed
RRID
AB_2565200 (BioLegend Cat. No. 917701)
Go To Top Version: 5    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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