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Purified anti-Gephyrin Antibody

Pricing & Availability
Clone
O93A6 (See other available formats)
Regulatory Status
RUO
Other Names
GPHN, Gephyrin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
O93A6_PURE_Gephyrin_Antibody_1_100918
Western blot of purified anti-Gephyrin antibody (clone O93A6). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O93A6_PURE_Gephyrin_Antibody_1_100918
    Western blot of purified anti-Gephyrin antibody (clone O93A6). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O93A6_PURE_Gephyrin_Antibody_2_100918
    IHC staining of purified anti-Gephyrin antibody (clone O93A6) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
  • O93A6_PURE_Gephyrin_Antibody_3_100918
    IHC staining of purified anti-Gephyrin antibody (clone O93A6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
  • O93A6_PURE_Gephyrin_Antibody_4_100918
    IHC staining of purified anti-Gephyrin antibody (clone O93A6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
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856101 25 µg £73
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856102 100 µg £189
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Description

Gephyrin (GPHN) is a scaffold protein that anchors glycine and GABA receptors to the tubulin cytoskeleton in the postsynaptic terminal of inhibitory synapses. Gephyrin's function as a scaffolding protein is regulated at transcriptional and translational levels, which impact the formation and plasticity of GABAergic synapses, as well as the overall strength of GABAergic transmission. Low levels of gephyrin in temporal lobe correspond to reduced inhibitory signals and correlate with temporal lobe epilepsy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant gephyrin expressed in human HEK293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783450 (BioLegend Cat. No. 856101)
AB_2783450 (BioLegend Cat. No. 856102)

Antigen Details

Structure
Gephyrin is a 736 amino acid protein with an apparent molecular mass of ~ 93 kD.
Distribution

Tissue Distribution: Nervous system, endocrine system, and gastrointestinal tract.
Cellular Distribution: Plasma membrane, cytoskeleton, and cytosol.

Function
Gephyrin is a scaffolding protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton.
Interaction
Homodimer and homo-oligomer, interacts with GLRB, GABARAP, GABRA3, GLRB.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Alvarez FJ. 2017. Brain Res. Bull. 129:50.
  2. Tyagarajan SK, Fritschy JM. 2014. Nat. Rev. Neurosci. 15:141-56.
  3. Zacchi P, et al. 2014. Front Cell. Neurosci. 8:103.
Gene ID
10243 View all products for this Gene ID 268566 View all products for this Gene ID 64845 View all products for this Gene ID
UniProt
View information about Gephyrin on UniProt.org
Go To Top Version: 1    Revision Date: 10/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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