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Purified anti-GM130 Antibody

Pricing & Availability
Clone
W18248A (See other available formats)
Regulatory Status
RUO
Other Names
Golgin A2, Golgin subfamily A member 2, 130 kD cis-Golgi matrix protein, Golgin 95, GOLGA2, SY11 Protein
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W18248A_PURE_GM130_Antibody_1_100120.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.25 µg/mL purified anti-GM130 antibody (clone W18248A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18248A_PURE_GM130_Antibody_1_100120.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.25 µg/mL purified anti-GM130 antibody (clone W18248A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18248A_PURE_GM130_Antibody_2_100120.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL either purified rat IgG2b, κ isotype control antibody (Cat. No. 400602) (panel A) or purified anti-GM130 antibody (clone W18248A) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • W18248A_PURE_GM130_Antibody_3_100120.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg purified rat IgG2b, κ isotype control antibody (Cat. No. 400601) or purified anti-GM130 antibody (clone W18248A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody raised against a separate epitope of GM130. Lane M: Molecular weight marker.
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937001 25 µg £70
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937002 100 µg £174
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Description

GM130 is a structural cis-Golgi protein that plays an essential role in vesicular transport. Mitotic fragmentation of the Golgi apparatus occurs through the CDK1-dependent regulation of the physical interaction between GM130 and the vesicle docking protein p115. Biochemical or genetic disruption of GM130 results in defective Golgi assembly. GM130 also plays a role in activating TPX2, a spindle assembly factor, to produce microtubule nuclei surrounding the Golgi, which are then directly bound by GM130 during mitotic events. Aberrant GM130 expression has been described in multiple malignancies where cells show altered Golgi structure and loss of polarity, lending to increased migration and invasion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human GM130 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse GM130 by western blot.

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol. Both methods were compatible with GM130 staining.

RRID
AB_2888895 (BioLegend Cat. No. 937001)
AB_2888895 (BioLegend Cat. No. 937002)

Antigen Details

Distribution

Cis-Golgi/Ubiquitously expressed

Function
Golgi structural protein
Biology Area
Cell Biology, Cell Structure
Molecular Family
Microtubules, Organelle Markers
Antigen References

1. Nakamura N, et al. 1997. Cell. 89:445.
2. Lowe M, et al. 1998. Cell. 94:783.
3. Wei JH, et al. 2015, Cell. 162:1.

Gene ID
2801 View all products for this Gene ID
UniProt
View information about GM130 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/01/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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