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Purified anti-Calnexin Antibody

Pricing & Availability
Clone
W17077C (See other available formats)
Regulatory Status
RUO
Other Names
CANX, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17077C_PURE_Calnexin_Antibody_1_022118
Whole cell lysates (15 µg protein) from HeLa (lane 1), HepG2 (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL), 1:2000 (0.25 µg/mL) and 1:5000 (0.1 µg/mL) purified anti-Calnexin antibody, clone W17077C or competitor’s antibody used at manufactures recommended concentration. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) for clone W17077C or 1:3000 diluted HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17077C_PURE_Calnexin_Antibody_1_022118
    Whole cell lysates (15 µg protein) from HeLa (lane 1), HepG2 (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL), 1:2000 (0.25 µg/mL) and 1:5000 (0.1 µg/mL) purified anti-Calnexin antibody, clone W17077C or competitor’s antibody used at manufactures recommended concentration. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) for clone W17077C or 1:3000 diluted HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17077C_PURE_Calnexin_Antibody_2_022118
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) 1:250 (2 µg/ml) purified rat IgG2a, κ isotype control antibody (Cat. No. 400502) or 1:250 (2 µg/ml, B) and 1:1000 (0.5 µg/ml, C) anti-Calnexin antibody (clone W17077C) overnight at 4°C followed by Alexa Fluor® 488 (green) conjugated goat anti-rat IgG (Cat. No. 405418) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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699401 25 µg £70
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699402 100 µg £174
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Description

Calnexin is a type I integral ER transmembrane chaperone that interacts with the oligosaccharide Glc1Man9GlcNAc2 on the glycoproteins and possesses binding sites for ATP and calcium.  Together with its soluble homolog calreticulin and the thiol oxidoreductase ERp57, calnexin was found to participate in the folding and quality control of newly synthesized N-linked glycoproteins. Calnexin has calcium-binding capacity and is involved in the regulation of calcium homeostasis. The potential calnexin binding substrates include MHC class I molecules, ion channel proteins, and the cystic fibrosis transmembrane conductance regulator (CFTR). Mutations on calnexin have been implicated in several coagulation disorders and cystic fibrosis. Calnexin-deficient mice exhibit severe motor problems and growth defects.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Calnexin recombinant protein (161-410 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-1:5000 diluted). For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The apparent molecular weight of Calnexin may differ based on the gel system used: in Tris-Glycine gels, a molecular weight of ~90 kDa was observed by WB testing, while in Bis-Tris gels an apparent molecular weight of ~75 kDa was observed.

Product Citations
  1. Tábara LC, et al. 2022. Brain. 145:3095. PubMed
  2. Hugendieck G, et al. 2023. J Extracell Vesicles. e12338:12. PubMed
  3. Antonicka H, et al. 2020. Cell Metabolism. 32(3):479-497.e9. PubMed
  4. Zhang Y, et al. 2021. J Extracell Vesicles. e12073:10. PubMed
  5. Aaltonen MJ, et al. 2022. Life Sci Alliance. 5:. PubMed
  6. Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
RRID
AB_2728519 (BioLegend Cat. No. 699401)
AB_2728519 (BioLegend Cat. No. 699402)

Antigen Details

Structure
592 amino acids with a predicted molecular weight of 67.6 kD. Type I transmembrane protein. Contains an N-terminal ER lumenal domain and a C-terminal cytoplasmic domain.
Distribution

Endoplasmic reticulum membrane.

Function
Calnexin is an ER transmembrane chaperone protein and functions in the folding of newly synthesized glycoprotein and quality control of misfolded proteins.
Interaction
Interacts with MAPK3/ERK1, KCNH2, SGIP1, SERPINA2P/SERPINA2, PPIB, and ZNRF4.
Biology Area
Cell Biology
Antigen References
  1. Lamriben L, et al. 2016. Traffic. 17:308.
  2. Kuang XL, et al. 2014. J. Neurosci. Res. 92:1319.
  3. Lederkremer GZ. 2009. Curr. Opin. Struct. Biol. 515-23.
  4. Beauregard PB, et al. 2009. J. Cell Sci. 122:1342.
  5. Rosser MF, et al. 2008. Mol. Biol. Cell. 4570-9.
  6. Caramelo JJ, Parodi AJ. 2008. J. Biol. Chem. 283:10221.
Gene ID
821 View all products for this Gene ID
UniProt
View information about Calnexin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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