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Purified anti-Granzyme B Antibody

Pricing & Availability
Clone
12F9B65 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, Cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, Aspase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
12F9B65_Purified_Granzyme_B_Antibody_WB_100614
5 ng of recombinant, His-thioredoxin tagged mouse Granzyme B was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-mouse Granzyme B (clone 12F9B65) antibody. Proteins were visualized using an anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 12F9B65_Purified_Granzyme_B_Antibody_WB_100614
    5 ng of recombinant, His-thioredoxin tagged mouse Granzyme B was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-mouse Granzyme B (clone 12F9B65) antibody. Proteins were visualized using an anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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662801 25 µg £70
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662802 100 µg £206
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Description

Granzyme B is a serine protease expressed by cytotoxic T cells (CTL) and NK cells. Its main function is to induce cell death to eliminate harmful targets such as allogeneic, virally infected, and tumor cells. This is evident by the fact that CTLs from mice deficient of granzyme B exhibit a profound defect in inducing rapid DNA fragmentation and apoptosis in target cells. Following receptor-mediated conjugate formation between CTL or NK and their target cell, granzyme B enters the target via endocytosis, and subsequently activates multiple protein substrates to induce apoptosis. Most circulating CD56+ and CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzyme A and B. In contrast, few circulating CD4+ T cells express granzymes A or B. Activation of CD8+ and CD4+ T cells induces substantial expression of granzyme B, but not granzyme A. Besides CTL and NK, evidence has shown that the distribution of human granzyme B has a broader spectrum of cells including CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, and B cells. Although its role in cytotoxic lymphocyte-mediated apoptosis is well established, granzyme B can also degrade extracellular matrix proteins and alter inflammation, if present in the extracellular milieu. These findings suggest that granzyme B can function as an activation molecule with potentially important immunoregulatory functions. In addition, it was shown that expression of granzyme B is elevated in acute coronary syndrome and acute myocardial infarction, indicating that granzyme B could be a factor involved in cardiovascular diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse Granzyme B recombinant protein (17-247 aa) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Anthony SM, et al. 2021. eLife. 10:00. PubMed
RRID
AB_2564373 (BioLegend Cat. No. 662801)
AB_2564373 (BioLegend Cat. No. 662802)

Antigen Details

Structure
227 amino acids with predicted molecular weight of 25.3 kD in mature form.
Distribution
Secreted.
Function
Granzyme B can induce target cell apoptosis by activating caspase independent pathways. It is induced in CD8+ T cells with ConA/ IL-2 and CD4+ T cells with anti CD3/CD28 or CD3/CD46.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 139:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6924.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 18:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.

Gene ID
14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org
Go To Top Version: 1    Revision Date: 10/06/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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