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Purified anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
GL3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ
Isotype
Armenian Hamster IgG
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Product Citations
publications
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118101 100 µg £81
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6J intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
  2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
  3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
  4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
  5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
  6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
  8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
  9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
Product Citations
  1. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  2. Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
  3. Díaz NM, et al. 2020. Invest Ophthalmol Vis Sci. 61:37:00. PubMed
  4. Belle A, et al. 2012. J Immunol. 188:462. PubMed
  5. Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed
  6. Waickman AT, et al. 2017. Cytokine. 99:266. PubMed
  7. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  8. Álvarez-Salamero C, et al. 2020. PLoS Biol. 18:e3000646. PubMed
  9. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  10. Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed
  11. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
  12. Shapiro MJ, et al. 2019. J Immunol. 202:2287. PubMed
  13. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  14. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  15. Yoshizaki T, et al. 2019. Sci Rep. 9:17067. PubMed
  16. Kasten K, et al. 2010. Infect Immun. 78:4714. PubMed
  17. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  18. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  19. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  20. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  21. Castro–Dopico T, et al. 2020. Cell Reports. 32(1):107857. PubMed
  22. Lopes N, et al. 2021. Nat Immunol. 22:179. PubMed
  23. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  24. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  25. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  26. Bréart B, et al. 2011. J Exp Med. 208:1267. PubMed
  27. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  28. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  29. Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
  30. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  31. Stadanlick J, et al. 2011. J Immunol. 187:664. PubMed
  32. Ma Z, et al. 2021. Cancer Med. 10:5358. PubMed
  33. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
RRID
AB_313826 (BioLegend Cat. No. 118101)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex.
Distribution

T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.

Function
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand/Receptor
Some bacterial or tumor antigens bound to MHC class I.
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Skarstein K, et al. 1995. Immunology. 81:497.
  2. Harrison LC, et al. 1996. J Exp Med. 184:2167.
  3. Wildner G, et al. 1996. Eur J Immunol. 26:2140.
  4. Brandes M, et al. 2005. Science. 309:264.
Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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