- Clone
- K13/16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- C3a, C3a desarg, C3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
518101 | 50 µg | £70 | ||||
518102 | 500 µg | £221 |
C3, complement component 3, is a 195 kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human C3a
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from 39 to 2500 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The K13/16 antibody reacts with human C3a, C3a(desArg) and C3. The K13/16 antibody can neutralize the bioactivity of natural or recombinant C3a.
ELISA Capture: The purified K13/16 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated D17/1 antibody (Cat. No. 518002) as the detecting antibody.
Neutralization: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human C3a/C3a(desArg)/C3 bioactivity. -
Application References
(PubMed link indicates BioLegend citation) -
- Oppermann M, et al. 1991. Kidney Int. 40:939947.
- Oppermann M, et al. 1992. Immunopharm. 24:119134.
- Elsner J, et al. 1994. Blood 83:3324.
- RRID
-
AB_10899410 (BioLegend Cat. No. 518101)
AB_10899410 (BioLegend Cat. No. 518102)
Antigen Details
- Ligand/Receptor
- C3a bind to C3a receptor, C3a desArg bind to C5L2 (GPR77)
- Biology Area
- Cell Biology, Complement, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
1. de Bruijn MH, et al. 1985. P. Natl. Acad. Sci. USA 82:708.
2. Hugli TE, et al. 1975. J. Biol. Chem. 250:8293.
3. Oxvig C, et al. 1995. J. Biol. Chem. 270:13645. - Gene ID
- 718 View all products for this Gene ID
- UniProt
- View information about C3a/C3adesArg/C3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All C3a/C3a(desArg)/C3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human C3a/C3a(desArg)/C3 | K13/16 | ELISA Capture,IP,WB |
Ultra-LEAF™ Purified anti-human C3a/C3a(desArg)/C3 | K13/16 | ELISA Capture,IP,WB,Neut |
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