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Purified anti-human CCL5 (RANTES) Antibody

Pricing & Availability
Clone
A19042E (See other available formats)
Regulatory Status
RUO
Other Names
SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine A5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A19042E_PURE_CCL5_Antibody_022024
Direct ELISA showing purified anti-human CCL5 (clone A19042E) bound to plate-immobilized recombinant human CCL5 (RANTES) (Cat. Nos. 580202, 580204, and 580206). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A19042E_PURE_CCL5_Antibody_022024
    Direct ELISA showing purified anti-human CCL5 (clone A19042E) bound to plate-immobilized recombinant human CCL5 (RANTES) (Cat. Nos. 580202, 580204, and 580206). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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620252 100 µg £288
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Description

RANTES is a CC chemokine and directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5/RANTES expression has been associated with a wide range of inflammatory disorders and pathologies. This cytokine has been associated as well with the induction or promotion of cancer (prostate and breast). In the latter situation, RANTES can be angiogenic and also increase the metastatic potential of cancer cells by encouraging cellular migration to sites of its production. CCL5/RANTES also inhibits HIV-1 entry and infection by competing with the virus for CCR5 binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant CCL5 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A19042E) could function as a capture antibody when paired with biotin anti-human CCL5 (RANTES) (Cat. No. 519703) (clone Poly5197) as the detection antibody and using Recombinant Human CCL5 (RANTES) (carrier-free) protein (Cat. Nos. 580202, 580204, and 580206) as the assay standard.

Antigen Details

Distribution

RANTES/CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. RANTES is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C.

Function
RANTES can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. RANTES modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by RANTES.
Interaction
RANTES/CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
Ligand/Receptor
CCL5 binds to CCR1, CCR3 and CCR5 receptors. In addition, CCL5 binds to CCR9; nevertheless, it does not induce calcium flux and therefore no chemotactic response.
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Schall, et al. 1990. Nature. 347:669-71.
  2. Cocchi F, et al. 1995. Science. 270:1811-5.
  3. Shahrara S, et al. 2006. J Immunology. 177:5077-87.
  4. Larrubia JR, et al. 2008. World J. Gastroenterol. 14:7149-59.
  5. Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920-7.
Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org
Go To Top Version: 1    Revision Date: 02/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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