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Ultra-LEAF™ Purified anti-human CCL5 Antibody

Pricing & Availability
Clone
A19042E (See other available formats)
Regulatory Status
RUO
Other Names
SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine A5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A19042E_ULEAF_CCL5_Antibody_072023
Recombinant human CCL5 (RANTES) (Cat. No. 580202) (black circles) induces the chemotaxis of mouse Baf3-hCCR5 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL5 (RANTES) (A19042E) (purple squares) neutralizes the chemotaxis of mouse Baf3- hCCR5 cells induced by recombinant human CCL5 (RANTES) in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 401507) (gray triangles) does not have the effect. ND50 range: 0.04 - 0.3 µg/mL
  • A19042E_ULEAF_CCL5_Antibody_072023
    Recombinant human CCL5 (RANTES) (Cat. No. 580202) (black circles) induces the chemotaxis of mouse Baf3-hCCR5 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL5 (RANTES) (A19042E) (purple squares) neutralizes the chemotaxis of mouse Baf3- hCCR5 cells induced by recombinant human CCL5 (RANTES) in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 401507) (gray triangles) does not have the effect. ND50 range: 0.04 - 0.3 µg/mL
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620253 100 µg £288
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620254 1 mg £1365
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CCL5 is a CC chemokine and directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5/RANTES expression has been associated with a wide range of inflammatory disorders and pathologies. This cytokine has been associated as well with the induction or promotion of cancer (prostate and breast). In the latter situation, RANTES can be angiogenic and also increase the metastatic potential of cancer cells by encouraging cellular migration to sites of its production. CCL5/RANTES also inhibits HIV-1 entry and infection by competing with the virus for CCR5 binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL5 (RANTES)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis of mouse Baf3- hCCR5 cells induced by recombinant human CCL5 (RANTES) (Cat. No. 580202). ND50 range: 0.04 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068220 (BioLegend Cat. No. 620253)
AB_3068220 (BioLegend Cat. No. 620254)

Antigen Details

Structure
Cytokines/Chemokines
Distribution

CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. RANTES is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C

Function
CCL5 can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. RANTES modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by RANTES.
Interaction
RANTES/CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
Ligand/Receptor
CCL5 binds to CCR1, CCR3 and CCR5 receptors. In addition, CCL5 binds to CCR9; nevertheless, it does not induce calcium flux and therefore no chemotactic response.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Schall, et al. 1990. Nature. 347:669-671.
  2. Cocchi F, et al. 1995. Science. 270:1811-1815.
  3. Shahrara S, et al. 2006. J. Immunology. 177:5077-5087.
  4. Larrubia JR, et al. 2008. World J. Gastroenterol. 14:7149-7159.
  5. Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920-1927.
Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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