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Purified anti-human CD120b Antibody

Pricing & Availability
Clone
3G7A02 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF1B, p75, p75TNFR, TNFR2, TNFR80, TBPII
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3G7A02_Purified_CD120b_Antibody_FC_051413
Human peripheral blood lymphocytes were stained with purified anti-human CD120b (clone 3G7A02) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by biotinylated anti-rat IgG and Sav-PE.
  • 3G7A02_Purified_CD120b_Antibody_FC_051413
    Human peripheral blood lymphocytes were stained with purified anti-human CD120b (clone 3G7A02) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by biotinylated anti-rat IgG and Sav-PE.
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358402 100 µg £77
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Description

CD120b, also known as TNFRII or TNFRSF1B, belongs to the TNF receptor superfamily and can interact with ligands TNF-α and TNF-β (also known as LT-α). It is a 75 kD type I transmembrane protein expressed on lymphocytes, monocytes, and granulocytes. When present in a heterocomplex with CD120a, it induces the recruitment of anti-apoptotic molecules, c-IAP1 and c-IAP2 that possess E3 ubiquitin ligase activity. Mutations in CD120b are associated with TNF-associated periodic syndrome (TRAPS) or periodic fever syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial CD120b recombinant protein expressed in 293E cells (22-211aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Minshawi F, et al. 2019. Sci Rep. 0.509027778. PubMed
  2. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
RRID
AB_2562150 (BioLegend Cat. No. 358402)

Antigen Details

Structure
75 kD, type I transmembrane protein, member of the TNF-receptor superfamily, forms a heterocomplex with TNFRI
Distribution

Expressed on cells of hematopoietic lineage including monocytes, lymphocytes, and granulocytes

Function
When complexed with TNFRI, recruits anti-apoptotic molecules c-IAP1 and c-IAP2 that possess ubiquitin ligase activity; cysteine rich extracellular region
Interaction
Interacts with TTRAP and TRAF2
Ligand/Receptor
TNF-α and TNF-β (LT-α)
Cell Type
Granulocytes, Lymphocytes, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Transcription Factors
Molecular Family
CD Molecules
Antigen References

1. Andrews JS, et al. 1990. J. Immunol. 144:2582.
2. Nophar Y, et al. 1990. EMBO J. 9:3269.
3. Kohno T, et al. 1990. Proc. Natl. Acad. Sci. USA 87:8331.
4. Tartaglia LA, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9292.
5. Hijdra D, et al. 2012. J. Inflamm. (Lond). 9:38.
6. Chen X, et al. 2013. J. Immunol. 190:1076.

Gene ID
7133 View all products for this Gene ID
UniProt
View information about CD120b on UniProt.org
Go To Top Version: 1    Revision Date: 06/07/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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