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Purified anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
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Product Citations
publications
6H6_PURE_CD123_Antibody_1_091924
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cyanine5
  • 6H6_PURE_CD123_Antibody_1_091924
    Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cyanine5
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306002 100 µg £70
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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Application References

(PubMed link indicates BioLegend citation)
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  2. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  3. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  4. Laggner M, et al. 2022. EBioMedicine. 81:104093. PubMed
  5. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  6. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  7. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  8. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  9. Parackova Z, et al. 2020. Sci Rep. 0.759027778. PubMed
  10. Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
  11. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  12. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  13. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  14. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  15. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  16. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  17. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  18. Chen S, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  19. Olin A, et al. 2018. Cell. 174:1277. PubMed
  20. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  21. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  22. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  23. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  24. Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
  25. Martin-Gayo E, et al. 2010. Blood. 115:5366. PubMed
  26. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  27. Henrick BM, et al. 2021. Cell. . PubMed
  28. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  29. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  30. Liu SQ, et al. 2021. Cancer Immunol Res. 9:34. PubMed
  31. NULL, et al. 2022. Cell. 185:916. PubMed
  32. Saygin C, et al. 2021. Leukemia. 35:3406. PubMed
  33. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  34. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  35. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314576 (BioLegend Cat. No. 306002)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org
Go To Top Version: 4    Revision Date: 08/30/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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