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Purified anti-human CD170 (Siglec-5) Antibody

Pricing & Availability
Clone
1A5 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70443
Other Names
OBBP2, CD33L2, OB-BP2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1A5_PE_051311
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 1A5_PE_051311
    Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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352002 100 µg £157
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Description

CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Bonobo, Gorilla
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular region of Siglec-5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1A5 antibody cross-reacts with Siglec-144.  Additional reported applications (for the relevant formats) include: ELISA2.

Clone 1A5 cross-reacts to Gorilla2

Application References

(PubMed link indicates BioLegend citation)
  1. Cornish AL, et al. 1998. Blood 92:2123.
  2. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
  3. Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
  4. Angata T, et al. 2006. FASEB J. 20:1964.
  5. Avril T, et al. 2005. J. Biol. Chem. 280:19843.
Product Citations
  1. Tseng YW, et al. 2021. Front Cell Infect Microbiol. 613195:11. PubMed
RRID
AB_10896789 (BioLegend Cat. No. 352002)

Antigen Details

Structure
Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs
Distribution

Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells

Function
Inhibitory receptor, cell adhesion
Interaction
SHP-1, SHP-2
Ligand/Receptor
α2,3- and α2,6-linked sialic acid, glycophorin A
Cell Type
B cells, Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Soto PC, et al. 2010. J. Immunol. 184:4185.
2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691.
3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.

Gene ID
8778 View all products for this Gene ID
UniProt
View information about CD170 on UniProt.org
Go To Top Version: 3    Revision Date: 08/15/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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