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Purified anti-human CD328 (Siglec-7) Antibody

Pricing & Availability
Clone
S7.7 (See other available formats)
Regulatory Status
RUO
Other Names
QA79 membrane protein, Adhesion inhibitory receptor molecule 1, p75/AIRM1, D-siglec
Isotype
Mouse IgG1, κ
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Product Citations
publications
a-S7-7_PE_CD328_Antibody_1_102324
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cyanine5.5 and purified S7.7 conjugated to PE
  • a-S7-7_PE_CD328_Antibody_1_102324
    Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cyanine5.5 and purified S7.7 conjugated to PE
  • b-S7dot7_PE_0305102.jpg
    Human peripheral blood monocytes stained with purified S7.7 conjugated to PE
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347702 100 µg £153
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Description

Siglec-7 is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs), also known as p75/AIRM1. It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Siglec-7 3T3 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Blocking2.

Application References

(PubMed link indicates BioLegend citation)
  1. Nicoll G, et al. 1999. J. Biol Chem. 274:34089.
  2. Hudak J, et al. 2014. Nat. Chem Biol. 10:69–75.
Product Citations
  1. Vuyyuru R, et al. 2011. Proc Natl Acad Sci U S A. 20707:108. PubMed
RRID
AB_2189411 (BioLegend Cat. No. 347702)

Antigen Details

Structure
Type I transmembrane protein, Siglec family member, 75 kD
Distribution

Monocytes, NK cells

Function
Inhibitory receptor of NK cells
Interaction
After tyrosine phosphorylation interacts with PTPN6 and SHP-1
Ligand/Receptor
Sialylated glycans (with α,8 disialyl and α2,6 sialyl residues)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Avril T, et al. 2006. Infect Immun. 74:4133.
2. Avril T, et al. 2006. J. Leukoc. Biol. 80:787.
3. Orr SJ, et al. 2007. J. Biol Chem. 282:3418.
4. Brunetta E, et al. 2009. Blood 114:3822.

Gene ID
27036 View all products for this Gene ID
UniProt
View information about CD328 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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