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Purified anti-human CD57 Antibody

Pricing & Availability
Clone
HNK-1 (See other available formats)
Regulatory Status
RUO
Other Names
HNK-1, NK-1, Leu-7
Isotype
Mouse IgM, κ
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Product Citations
publications
HNK-1_Purified_CD57_Antibody_FC_1_081413.jpg
Human peripheral blood lymphocytes were stained with CD8 APC and purified CD57 (clone HNK-1) (bottom) or purified mouse IgM, κ isotype control (top), followed with anti-mouse IgM PE.
  • HNK-1_Purified_CD57_Antibody_FC_1_081413.jpg
    Human peripheral blood lymphocytes were stained with CD8 APC and purified CD57 (clone HNK-1) (bottom) or purified mouse IgM, κ isotype control (top), followed with anti-mouse IgM PE.
  • HNK-1_Purified_CD57_Antibody_FC_2_081413.jpg
  • HNK-1_Purified_CD57_Antibody_FC_3_010918
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate-Buffered Saline 1X (pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for 5 minutes and blocked with 5% FBS and 0.2% Gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-CD57 (clone HNK-1) overnight. On the next day, tissue was incubated with Alexa Flour® 647 anti-mouse IgM (Clone RMM-1) antibody (red), Alexa Flour® 594 anti-TUBB3 (Clone AA10) antibody (blue) and Brilliant Violet 421™ anti-GFAP Antibody (green) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
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359602 100 µg £94
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Description

CD57, also known as HNK-1, NK-1, and Leu-7 is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Membrane extract of human lymphoblastoid cell line HSB-2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshihara Y, et al. 1991. J. Cell Biol. 115:731. (WB)
  2. Abo T, et al. 1981. J. Immunol. 127:1024.
  3. Abo T, et al. 1982. J. Immunol. 129:1752.
  4. Abo T, et al. 1982. J. Immunol. 129:1758.
Product Citations
  1. Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed
  2. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  3. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  4. Arunachalam PS, et al. 2021. Nature. 596:410. PubMed
  5. Neidleman J, et al. 2020. Elife. 9:00. PubMed
  6. Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
  7. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  8. Neidleman J, et al. 2021. Elife. 10:. PubMed
  9. Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed
  10. Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed
  11. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_2562403 (BioLegend Cat. No. 359602)

Antigen Details

Structure
Oligosaccharide antigenic determinant present on a variety of proteins, lipids, and chrondroitin sulfate proteoglycans, 100-115 kD. The antigen is conserved across species.
Distribution

CD57 antigen is expressed on a subset of peripheral blood lymphocytes including a subset of NK cells and a subset of CD8+ T cells. Also expressed on neural cells and striated muscle.

Function
Unknown function, may be involved in cell-matrix interactions.
Ligand/Receptor
Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.

Gene ID
27087 View all products for this Gene ID
UniProt
View information about CD57 on UniProt.org
Go To Top Version: 1    Revision Date: 08/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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