- Clone
- 3B2/TA8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD99, MIC2, HBA71, MSK5X, E2 antigen
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3B2slashTA8_PURE_CD99_Antibody_031816 3B2slashTA8_PURE_CD99_Antibody_031816](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3B2slashTA8_PURE_CD99_Antibody_031816.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with purified CD99 (clone 3B2/TA8, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE. -
Human paraffin-embedded pancreas tissue slice was stained with purified anti-human CD99 (clone 3B2/TA8) antibody overnight followed by Alexa Fluor® 488 goat anti-mouse secondary antibody (green). The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
371302 | 100 µg | £81 |
CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human thymus
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
-
Application References
(PubMed link indicates BioLegend citation) -
- Waclavicek M, et al. 1998. J. Immunol. 161:4671.
- Pickl W, et al. 2001. J. Virol. 75:7175.
- RRID
-
AB_2572177 (BioLegend Cat. No. 371302)
Antigen Details
- Structure
- Surface glycoprotein, type I transmembrane protein, 32kD.
- Distribution
-
T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.
- Function
- Leukocyte migration, T-cell adhesion, T-cell death- caspase independent, and spontaneous rosette formation with erythrocytes.
- Interaction
- Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
- Cell Type
- Leukemia, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Gelin C, et al. 1989. EMBO. 8:3253.
2. Goodfellow PJ, et al. 1986. Science 234:740.
3. Pettersen RD, et al. 2001. J. Immunol. 166:4931. - Gene ID
- 4267 View all products for this Gene ID
- UniProt
- View information about CD99 on UniProt.org
Related FAQs
Other Formats
View All CD99 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD99 | 3B2/TA8 | FC,IHC-P |
FITC anti-human CD99 | 3B2/TA8 | FC |
PE anti-human CD99 | 3B2/TA8 | FC |
APC anti-human CD99 | 3B2/TA8 | FC |
Brilliant Violet 421™ anti-human CD99 | 3B2/TA8 | FC,IHC-P |
PE/Cyanine7 anti-human CD99 | 3B2/TA8 | FC |
PerCP/Cyanine5.5 anti-human CD99 | 3B2/TA8 | FC |
TotalSeq™-A0845 anti-human CD99 | 3B2/TA8 | PG |
TotalSeq™-C0845 anti-human CD99 | 3B2/TA8 | PG |
TotalSeq™-B0845 anti-human CD99 | 3B2/TA8 | PG |
TotalSeq™-D0845 anti-human CD99 | 3B2/TA8 | PG |
PE anti-human CD99 | 3B2/TA8 | FC |
GMP PE anti-human CD99 | 3B2/TA8 | FC |
FITC anti-human CD99 | 3B2/TA8 | FC |
Spark Red™ 718 anti-human CD99 (Flexi-Fluor™) | 3B2/TA8 | FC |
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/hec2_FITC_CD99_Antibody_020320.png)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/HIP8_Purified_062707.jpg)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/hi9a.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD99
Human peripheral blood lymphocytes were stained with purifie... Human paraffin-embedded pancreas tissue slice was stained wi... -
FITC anti-human CD99
Human peripheral blood lymphocytes were stained with CD99 FI... -
PE anti-human CD99
Human peripheral blood lymphocytes were stained with CD99 (c... -
APC anti-human CD99
Human peripheral blood lymphocytes were stained with CD99 AP... -
Brilliant Violet 421™ anti-human CD99
Human paraffin-embedded pancreas tissue slices were prepared... Human peripheral blood lymphocytes were stained with CD99 Br... -
PE/Cyanine7 anti-human CD99
Human peripheral blood lymphocytes were stained with CD99 PE... -
PerCP/Cyanine5.5 anti-human CD99
Human peripheral blood lymphocytes were stained with CD99 Pe... -
TotalSeq™-A0845 anti-human CD99
-
TotalSeq™-C0845 anti-human CD99
-
TotalSeq™-B0845 anti-human CD99
-
TotalSeq™-D0845 anti-human CD99
-
PE anti-human CD99
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human CD99
Typical results from human peripheral blood lymphocytes stai... -
FITC anti-human CD99
Typical results from human peripheral lymphocytes stained ei... -
Spark Red™ 718 anti-human CD99 (Flexi-Fluor™)
Follow Us