- Clone
- 3B2/TA8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD99, MIC2, HBA71, MSK5X, E2 antigen
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human thymus
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Waclavicek M, et al. 1998. J. Immunol. 161:4671.
- Pickl W, et al. 2001. J. Virol. 75:7175.
- RRID
-
AB_3106310 (BioLegend Cat. No. 285046)
Antigen Details
- Structure
- Surface glycoprotein, type I transmembrane protein, 32kD.
- Distribution
-
T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.
- Function
- Leukocyte migration, T-cell adhesion, T-cell death- caspase independent, and spontaneous rosette formation with erythrocytes.
- Interaction
- Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
- Cell Type
- Leukemia, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Gelin C, et al. 1989. EMBO. 8:3253.
2. Goodfellow PJ, et al. 1986. Science 234:740.
3. Pettersen RD, et al. 2001. J. Immunol. 166:4931. - Gene ID
- 4267 View all products for this Gene ID
- UniProt
- View information about CD99 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD99 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD99 | 3B2/TA8 | FC,IHC-P |
FITC anti-human CD99 | 3B2/TA8 | FC |
PE anti-human CD99 | 3B2/TA8 | FC |
APC anti-human CD99 | 3B2/TA8 | FC |
Brilliant Violet 421™ anti-human CD99 | 3B2/TA8 | FC,IHC-P |
PE/Cyanine7 anti-human CD99 | 3B2/TA8 | FC |
PerCP/Cyanine5.5 anti-human CD99 | 3B2/TA8 | FC |
TotalSeq™-A0845 anti-human CD99 | 3B2/TA8 | PG |
TotalSeq™-C0845 anti-human CD99 | 3B2/TA8 | PG |
TotalSeq™-B0845 anti-human CD99 | 3B2/TA8 | PG |
TotalSeq™-D0845 anti-human CD99 | 3B2/TA8 | PG |
PE anti-human CD99 | 3B2/TA8 | FC |
GMP PE anti-human CD99 | 3B2/TA8 | FC |
FITC anti-human CD99 | 3B2/TA8 | FC |
Spark Red™ 718 anti-human CD99 (Flexi-Fluor™) | 3B2/TA8 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD99
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FITC anti-human CD99
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PE anti-human CD99
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APC anti-human CD99
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Brilliant Violet 421™ anti-human CD99
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PE/Cyanine7 anti-human CD99
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PerCP/Cyanine5.5 anti-human CD99
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TotalSeq™-A0845 anti-human CD99
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TotalSeq™-C0845 anti-human CD99
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TotalSeq™-B0845 anti-human CD99
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TotalSeq™-D0845 anti-human CD99
-
PE anti-human CD99
-
GMP PE anti-human CD99
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FITC anti-human CD99
-
Spark Red™ 718 anti-human CD99 (Flexi-Fluor™)
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