- Clone
- A16065C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- C-reactive protein (CRP), Pentraxin 1 (PTX1)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
696006 | 100 µg | £189 |
C-reactive protein (also known as CRP and Pentraxin 1 (PTX1)), is a member of pentraxin protein family. Similar to other pentraxins, CRP is a pattern recognition molecule that is able to recognize pathogens and promote their clearance. CRP tends to form a non-covalently linked pentamer with each subunit made up of two antiparallel β-sheets and a single short α-helix. It is characterized by the presence of a cleft that extends from the center of the subunit to its edge at the central pore of the pentamer structure. It has been demonstrated that CRP binds to the phosphocholine moieties expressed on the surface of dead or dying cells and some bacteria. This leads to the activation of the complement system and promotion of phagocytosis by macrophages to clear necrotic and apoptotic cells. CRP is secreted by hepatocytes and is a biomarker for inflammation. IL-6 has been shown to be an effective inducer of this protein. Besides binding to apoptotic cells, CRP also binds to a variety of molecules including C1q, bacterial polysaccharide, CD64, CD32, APCS, HDL, and fibronectin. In addition to participating in immune responses, CRP is also a prominent partaker in endothelial dysfunction and atherosclerosis. It was shown that CRP may exhibit direct proantherogenic effects by upregulating angiotensin type I receptor in smooth muscle cells and promoting their migration, proliferation, neointimal formation, and reactive oxygen species production. Mature human CRP shares 71% amino acid sequence homology with its mouse counterpart.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CRP/PTX1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 8 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For IHC-P, use 100 mM Tris, 10 mM EDTA, pH 9.0 for antigen retrieval buffer. Not recommended for use with Tris-buffered saline antigen retrieval buffer.
- RRID
-
AB_2686984 (BioLegend Cat. No. 696006)
Antigen Details
- Structure
- Monomer and homopentamer
- Distribution
-
Hepatocytes
- Function
- Induces expression of E-selectin and VCAM-1 expression in epithelial cells. CRP binds to target, initiates, and regulates complement activation. Induced by IL-1 and IL-6.
- Interaction
- Phosphocholine on the surface of apoptotic cells
- Ligand/Receptor
- Interacts with C1q, bacterial polysaccharide, CD64, CD32, APCS (PTX2), HDL, leptin, and fibronectin.
- Biology Area
- Cell Biology
- Antigen References
-
1. Ansar W, Ghosh S. 2013. Immunol. Res. 56:131.
2. Agrawal A, et al. 2009. Adv. Exp. Med. Biol. 653:98.
3. Garlanda C, et al. 2005. Annu. Rev. Immunol. 23:337.
4. Verma S, et al. 2004. Circulation 109:1914.
5. Wadham C, et al. 2004. Circulation 109:2116.
6. Li XA, et al. 1998. Biochem. Biophys. Res. Commun. 244:249.
7. Lu J, et al. 2012. Immunol. Rev. 250:230.
8. Chen K, et al. 2006. Nat. Med. 12:425. - Gene ID
- 1401 View all products for this Gene ID
- UniProt
- View information about CRP on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CRP (C-reactive protein) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-human CRP (C-reactive protein) | A16065C | Neut |
Purified anti-human CRP (C-reactive protein) | A16065C | WB,IHC-P |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Ultra-LEAF™ Purified anti-human CRP (C-reactive protein)
Immobilized recombinant human CRP at 2 µg/mL binds recombina... -
Purified anti-human CRP (C-reactive protein)
Total U87-MG cell lysates (negative control) and human serum... IHC staining using purified anti-CRP (C-reactive protein) an...
Follow Us