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Purified anti-human TMPRSS2 Antibody

Pricing & Availability
Clone
S20014A (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane protease serine 2
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_S20014A_PURE_TMPRSS2_Antibody_2_012822
Human colorectal adenocarcinoma cell line, Caco-2, was stained with purified anti-human TMPRSS2 (clone S20014A) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • 1_S20014A_PURE_TMPRSS2_Antibody_2_012822
    Human colorectal adenocarcinoma cell line, Caco-2, was stained with purified anti-human TMPRSS2 (clone S20014A) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • 2_S20014A_PURE_TMPRSS2_Antibody_1_012822_updated
    Human paraffin embedded colon tissue was subjected to heat-mediated antigen retrieval with sodium citrate and stained with 5 µg/mL purified anti-human TMPRSS2 (clone S20014A) (red) followed by Alexa Fluor® 555 anti-mouse IgG. Nuclei were counterstained with DAPI (blue). The image was captured by a 20X objective.
  • 3_S20014A_PURE_TMPRSS2_Antibody_3_020422
    The effect of anti-human TMPRSS2 (clone S20014A) on the ability of cells to migrate was tested by a Matrigel invasion assay. The human colon carcinoma cell line, Caco-2 was serum starved for 24h and seeded onto the top well of Corning® BioCoat Matrigel® Invasion Chamber. Nafamostat (25 µg/mL), mouse IgG1, κ isotype control antibody and untreated cells were used as controls. DMEM media with 20% serum was placed in the bottom well as chemoattractant. After 40h, cells that had migrated to the bottom of the membrane were counted.
  • 4_S20014A_PURE_TMPRSS2_Antibody_4_020422
    The effect of anti-human TMPRSS2 (clone S20014A) on TMPRSS2 protease activity was tested using a fluorescent protease assay. Recombinant TMPRSS2 was added to wells containing either S20014A, mouse IgG, κ or Nafamostat at 1 µg/mL each. Boc-Gln-Ala-ArgAMC, a protease substrate that exhibits fluorescence when cleaved by proteases, was added at a final concentration of 5 µM. Fluorescence was measured after 3h at 37°C. Protease activity was normalized to wells containing only PBS and Boc-Gln-Ala-ArgAMC.
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378402 100 µg £230
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Description

TMPRSS2 is a membrane-anchored protein that belongs to the serine protease family and is involved in many physiological and pathological processes. TMPRSS2 participates in proteolytic cascades and is important in normal physiological function of the prostate. Autocleavage leads to secretion of the protease domain. TMPRSS2 is involved in extracellular matrix disruption and metastasis of prostate cancer cells and activates several substrates, including pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14. TMPRSS2 promotes uptake of SARS-CoV-1, MERS-CoV, and SARS-CoV-2 viral particles of by proteolytic cleavage of ACE2 receptor and cleavage of coronavirus spike glycoproteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
huTMPRSS2 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

FC- Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Additional reported applications include: Flow cytometry.

RRID
AB_2910437 (BioLegend Cat. No. 378402)

Antigen Details

Structure
Single-pass type II membrane protein
Distribution

Cell membrane, activated by cleavage and secreted

Function
Hydrolase, protease, serine protease
Interaction
ACE2, HGF, F2RL1, ST14
Cell Type
Epithelial cells, Platelets
Biology Area
Cancer Biomarkers, Cell Biology, COVID-19
Molecular Family
Proteases
Antigen References
  1. Hoffman M, et al. 2020. Cell. 181:271-280.
  2. Yu J, et al. 2010. Cancer Cell. 17:443-54.
  3. Shen LW, et al. 2017. Biochimie. 142:1-10.
Gene ID
7113 View all products for this Gene ID
UniProt
View information about TMPRSS2 on UniProt.org
Go To Top Version: 1    Revision Date: 01/28/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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