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Purified anti-Lamin A/C Antibody

Pricing & Availability
Clone
WL4G10 (See other available formats)
Regulatory Status
RUO
Other Names
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WL4G10_Purified_Lamin_A-C_Antibody_1_041919.png
Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Lamin A/C Antibody, clone WL4G10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • WL4G10_Purified_Lamin_A-C_Antibody_1_041919.png
    Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Lamin A/C Antibody, clone WL4G10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • WL4G10_Purified_Lamin_A-C_Antibody_2_041919.png
    Total cell lysates (15 µg total protein) from Daudi (reduced expression control) and HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-Lamin A/C Antibody, clone WL4G10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted LMNA expression data was obtained from Human Protein Atlas.
  • WL4G10_Purified_Lamin_A-C_Antibody_3_041919.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5 µg/mL) of either Purified Mouse IgG1, κ Isotype Control Antibody (Cat. No. 401402, panel A) or Purified anti-Lamin A/C Antibody, Clone WL4G10 (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • WL4G10_Purified_Lamin_A-C_Antibody_4_062023
    Daudi cells (negative control, open histogram) or Jurkat cells (positive control, filled histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with purified anti-TAL1 (clone 4G8C07), or purified mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 401401), followed by PE goat anti-mouse IgG (Cat. No. 405307).
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600001 25 µg £70
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600002 100 µg £174
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Description

Lamins A and C are structural components of the lamina, a scaffold of proteins localized to the nuclear inner membrane, and contribute to nuclear membrane dynamics and chromatin structure. Mutations in the LMNA gene have been linked to multiple laminopathy disorders, including Hutchinson-Gilford progeria syndrome (HGPS), an autosomal dominant disorder characterized by the appearance of accelerated and dramatic aging. Patients with HGPS have a mutation in the LMNA gene that results in aberrant splicing and production of the toxic, truncated Lamin A protein progerin that functions in a dominant negative pattern.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST fused to C-terminal fragment of Lamin A (amino acid residues 422-664)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. For flow cytometric staining using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

WL4G10 does not recognize mouse Lamin A or C.

WL4G10 failed to immunoprecipitate Lamin A or C from HeLa lysates.

During PD testing for ICC applications, WL4G10 staining of PFA-fixed HeLa cells was compatible with both Methanol and Triton X-100 permeabilization methods; observed staining was slightly better with Triton X-100 permabilization.

RRID
AB_2810655 (BioLegend Cat. No. 600001)
AB_2810655 (BioLegend Cat. No. 600002)

Antigen Details

Structure
Lamin A is a 664 amino acid protein with a predicted molecular weight of 74 kD. Lamin C is a 572 amino acid protein with a predicted molecular weight of 65 kD.
Distribution

Ubiquitously expressed/Nucleoplasmic side of inner nuclear membrane

Function
Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
Cell Type
Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers, Western Blot Controls
Molecular Family
Nuclear Markers, Organelle Markers
Gene ID
4000 View all products for this Gene ID
UniProt
View information about Lamin A on UniProt.org
Go To Top Version: 2    Revision Date: 06/20/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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