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Purified anti-LAT Antibody

Pricing & Availability
Clone
LAT1111 (See other available formats)
Regulatory Status
RUO
Other Names
Linker for activation of T-cells, 36 kD phospho-tyrosine adaptor protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
LAT1111_1_Purified_LAT_Antibody_1_WB_060118
Total lysates (15 µg protein) from HEL, U87-MG and Daudi (Low expressed cells) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-LAT antibody, clone LAT1111. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • LAT1111_1_Purified_LAT_Antibody_1_WB_060118
    Total lysates (15 µg protein) from HEL, U87-MG and Daudi (Low expressed cells) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-LAT antibody, clone LAT1111. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • LAT1111_2_Purified_LAT_Antibody_ICFC_1_121714
  • LAT1111_2_Purified_LAT_Antibody_ICFC_2_121714
Cat # Size Price Quantity Check Availability Save
623901 25 µg £70
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623902 100 µg £150
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Description

LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant protein (cytoplasmic domain of human LAT)
Formulation
This LAT antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT.

Product Citations
  1. Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
RRID
AB_2133118 (BioLegend Cat. No. 623901)
AB_2133118 (BioLegend Cat. No. 623902)

Antigen Details

Structure
SH2 domains, CVRC motif; two isoforms 36 kD, 38 kD
Distribution

Lipid rafts, potential type III transmembrane protein, juxtanuclear intracellular compartment

Function
Links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression; required for T-cell development
Interaction
Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, cbl
Modification
Phosphorylation by ZAP-70, Syk, Palmitoylated
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Zhang W, et al. 1998. Cell. 92:83.
2. Zeyda M, et al. 2002. J. Biol. Chem. 277:28418.
3. Perez-Villar J, et al. 2002 Biochemistry. 41:10732.
4. Hartgroves L, et al. 2003 J. Biol. Chem. 278:20389.

Regulation
Phosphorylated when activated by TCR cross-linking, recruits signaling molecules, activation prevented by polyunsaturated fatty acids
Gene ID
27040 View all products for this Gene ID
UniProt
View information about LAT on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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