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Purified anti-LRRK2 Antibody (Previously Covance catalog# SIG-39840)

Pricing & Availability
Clone
MC.028.83.76.242 (See other available formats)
Regulatory Status
RUO
Other Names
Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 17, AURA17, DARDARIN, PARK8, RIPK7, ROCO2
Previously
Covance Catalog# SIG-39840
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_MC.028.83.76.242_PURE_LRRK2_IHCP_060518
IHC staining of purified anti-LRRK2 antibody (clone MC.028.83.76.242) on the formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 25 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_MC.028.83.76.242_PURE_LRRK2_IHCP_060518
    IHC staining of purified anti-LRRK2 antibody (clone MC.028.83.76.242) on the formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 25 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_MC0288376242_PURE_LRRK2_ELISA_060518
    Direct ELISA of purified ant-LRRK2 antibody (clone MC.028.83.76.242). Wells were coated with 100 ng of plate-immobilized recombinant human LRRK2 peptide or sAPP Beta peptide. The wells were then incubated with the primary antibody at 37°C for 60 minutes, followed by incubation with the horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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808201 100 µL £78
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Description

Leucine-rich repeat kinase 2 (LRRK2), is a member of the leucine-rich repeat kinase family. This protein also functions as a GTPase. Proteins with leucine-rich regions play a role in protein-protein interactions and mediate assembly of the cell's structural framework (cytoskeleton). Mutations in LRRK2 genes have been associated with late-onset Parkinson's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide immunogen comprising amino acids 2069-2087 of the LRRK2 protein in mice that did not express endogenous LRRK2 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 25 µg/mL is suggested. For Direct ELISA, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody was generated with support from The Michael J. Fox Foundation. The Michael J. Fox Foundation is dedicated to finding a cure for Parkinson's disease through an aggressively funded research agenda and to ensuring the development of improved therapies for those living with Parkinson's today. To learn more about MJFF, please visit www.michaeljfox.org.

Product Citations
  1. Fernández B, et al. 2022. J Parkinsons Dis. 12:1423. PubMed
  2. Wang X, et al. 2021. Sci Rep. 11:12900. PubMed
RRID
AB_2564739 (BioLegend Cat. No. 808201)

Antigen Details

Structure
This gene is a member of the leucine-rich repeat kinase family and is encoded by the PARK8 gene. The protein is present mainly in the cytoplasm but can be associated with the mitochondrial outer membrane.
Distribution

Tissue distribution: Lung, Kidney, parathyroid gland, CNS
Cellular distribution: cytoplasm, mitochondrion, nucleus, endoplasmic reticulum, lysosome, Golgi apparatus, endosome

Function
LRRK2 interacts with the C-terminal R2 RING finger domain of parkin. LRRK2 positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. Mutations in this gene have been associated with Parkinson disease.
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References
  1. Paisán-Ruíz C, et al. 2004. Neuron. 44(4):595-600.
  2. Alegre-Abarrategui J, et al. 2009. Hum. Mol. Genet. 18(21):4022-34.
  3. Gómez-Suaga P, et al. 2012. Hum. Mol. Genet. 21(3):511–25.
  4. Orenstein SJ, et al. 2013. Nat. Neurosci. 16(4):394–406.
  5. Cherra SJ, et al. 2013. Am. J. Pathol. 182(2):474–84. 
Gene ID
120892 View all products for this Gene ID
UniProt
View information about LRRK2 on UniProt.org

Other Formats

View All LRRK2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-LRRK2 MC.028.83.76.242 IHC-P,Direct ELISA
Go To Top Version: 6    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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