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Purified anti-mouse CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
10E5 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1 (platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
10E5_PURE_CD226_Antibody_1_011718
C57BL/6 mouse splenocytes were stained with CD8a APC and purified CD226 (clone 10E5, left) or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG2b FITC.
  • 10E5_PURE_CD226_Antibody_1_011718
    C57BL/6 mouse splenocytes were stained with CD8a APC and purified CD226 (clone 10E5, left) or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG2b FITC.
  • 10E5_PURE_CD226_Antibody_2_011718
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml CD226 (clone 10E5) purified and CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/ml of goat anti rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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128822 500 µg £254
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Description

CD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on CD4+ T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4+ cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Th1 polarized T cell clones
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
  2. Huang Z, et al. 2011. J Immunol. 186:3421. PubMed
Product Citations
  1. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  2. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
RRID
AB_2728146 (BioLegend Cat. No. 128822)

Antigen Details

Structure
65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.
Distribution

Expressed on majority of CD4+ and CD8+ T cells, NK cells, monocytes/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.

Function
Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation
Ligand/Receptor
CD112 and CD155
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tahara Hanaoka S, et al. 2006. Blood 107:1491
2. Shibuya K, et al. 2003. J. Exp. Med. 198:1829

Gene ID
225825 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org
Go To Top Version: 0    Revision Date: 01/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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