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Purified anti-mouse CD25 Antibody

Pricing & Availability
Clone
7D4/CD25 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, IL2RA, IL-2Ra, Ly-43, p55, Tac
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
7D4slashCD25_PURE_CD25_Antibody_1_091417
C57BL/6 mouse splenocytes were stimulated with Con A + IL-2 for 3 days and then stained with CD3 Alexa Fluor® 647 and purified CD25 (clone 7D4/CD25, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM-FITC.
  • 7D4slashCD25_PURE_CD25_Antibody_1_091417
    C57BL/6 mouse splenocytes were stimulated with Con A + IL-2 for 3 days and then stained with CD3 Alexa Fluor® 647 and purified CD25 (clone 7D4/CD25, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM-FITC.
  • 7D4slashCD25_PURE_CD25_Antibody_2_091417
  • 7D4slashCD25_PURE_CD25_Antibody_3_091417
    C57BL/6 mouse splenocytes stained with CD3 Alexa Fluor® 647 and purified CD25 (clone 7D4/CD25, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM-FITC.
  • 7D4slashCD25_PURE_CD25_Antibody_4_091417
Cat # Size Price Quantity Check Availability Save
154202 500 µg £157
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Description

CD25, also known as IL-2 low affinity receptor is a type I, 55 kDa, transmembrane glycoprotein. CD25 It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
HT-2 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, Depletion, IP - Reported in the literature, not verified in house 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP)
  2. Couper KN. 2007. J. Immunol. 178:4136. (Depletion)
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed
RRID
AB_2716173 (BioLegend Cat. No. 154202)

Antigen Details

Structure
Type I, 55 kD, transmembrane glycoprotein
Distribution

Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells

Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Huss DJ, et al. 2015. J. Immunol. 194:84.
2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056.
3. Codarri L, et al. 2007. J. Exp. Med. 204:1533.
4. Read S, et al. 2000. J. Exp. Med. 192:295.
5. Taniguchi T, et al. 1993. Cell 73:5. 

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD25 7D4/CD25 FC,Block,Depletion,IP
Go To Top Version: 0    Revision Date: 09/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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