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Purified anti-mouse CD8a Antibody

Pricing & Availability
Clone
S18018A (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
S18018A_PURE_CD8a_Antibody_012022
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL purified anti-mouse CD8a (clone S18018A) in 5% FBS overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG1 (clone MRG1-58) Alexa Fluor® 594 (red), 5 µg/mL of anti-mouse CD4 (clone GK1.5) Alexa Fluor® 488 (blue), and 5 µg/mL of anti-mouse/human CD45R/B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) at room temperature for 2 hours. The image was captured by 10X objective
  • S18018A_PURE_CD8a_Antibody_012022
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL purified anti-mouse CD8a (clone S18018A) in 5% FBS overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG1 (clone MRG1-58) Alexa Fluor® 594 (red), 5 µg/mL of anti-mouse CD4 (clone GK1.5) Alexa Fluor® 488 (blue), and 5 µg/mL of anti-mouse/human CD45R/B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) at room temperature for 2 hours. The image was captured by 10X objective
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164702 100 µg £45
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD8a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910346 (BioLegend Cat. No. 164702)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Ledbetter JA, et al. 1979. Immunol Rev. 47:63-90. 
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1.
  3. Takahashi K, et al. 1992. Proc Natl Acad Sci USA. 89:5557-61.
  4. Ledbetter JA, et al. 1981. J Exp Med. 153:1503-16. 
  5. Hata H, et al. 2004. J Clin Invest. 114:582-8. 
  6. Fan WY, et al. 2001. Exp Biol Med. 226:1045-50. 
  7. Shih FF, et al. 2006. J Immunol. 176:3438-48. 
  8. Kamimura D, et al. 2006. J Immunol. 177:306-14.
  9. Bouwer HGA, et al. 2006. Proc Natl Acad Sci USA. 103:5102-7.
  10. Kao C, et al. 2005. Int Immunol. 17:1607-17. 
  11. Ko SY, et al. 2005. J Immunol. 175:3309-17. 
  12. Rasmussen JW, et al. 2006. Infect Immun. 74:6590-8.
Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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