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Purified anti-NOS2 Antibody

Pricing & Availability
Clone
5C1B52 (See other available formats)
Regulatory Status
RUO
Other Names
Nitric oxide synthase 2, nitric oxide synthase 2A, NOS2A, HEP-NOS, inducible nitric oxide synthase (iNOS)
Isotype
Mouse IgG1, κ
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Product Citations
publications
5C1B52_PURE_NOS2_Antibody_1_WB_092616
Total cell lysate from human HT-29 cells (lane 1), HT-29 cells treated with IL-1β, TNFα, and IFNγ (lane 2), HCT-116 cells (lane 3), HCT-116 cells treated with IL-1β, TNFα, and IFNγ (lane 4), mouse RAW264.7 cells (lane 5), and RAW264.7 cells treated with LPS (lane 6), were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-NOS2 (clone 5C1B52) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
  • 5C1B52_PURE_NOS2_Antibody_1_WB_092616
    Total cell lysate from human HT-29 cells (lane 1), HT-29 cells treated with IL-1β, TNFα, and IFNγ (lane 2), HCT-116 cells (lane 3), HCT-116 cells treated with IL-1β, TNFα, and IFNγ (lane 4), mouse RAW264.7 cells (lane 5), and RAW264.7 cells treated with LPS (lane 6), were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-NOS2 (clone 5C1B52) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
  • 5C1B52_PURE_NOS2_Antibody_2_IF_092616
    RAW264.7 cells (left panel) and RAW264.7 cells treated with LPS (right panel) were fixed with 2% paraformaldehyde for 15 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.0 µg/ml purified anti-NOS2 (clone 5C1B52) antibody for one hour, followed by staining with Alexa Fluor® 488 con
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690902 100 µg £174
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Description

Nitric Oxide Synthase 2 (NOS2), also known as inducible NOS (iNOS), contains an N-terminal oxygenase domain and a C-terminal reductase domain, and functions to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a reactive free radical which acts as a biologic messenger with diverse functions throughout the body, such as neurotransmission, antimicrobial, and antitumor activity. NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. NOS2 also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such as COX2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NOS2 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Li T, et al. 2023. Adv Sci (Weinh). 10:e2206517. PubMed
  2. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
RRID
AB_2629826 (BioLegend Cat. No. 690902)

Antigen Details

Structure
1153 amino acids with a predicted molecular weight of 131 kD. Contains a flavodoxin-like domain and a FAD-binding FR-type domain.
Distribution

Cytoplasm.

Function
NOS2 is a nitric oxide synthase and produces large quantity of NO in response to the stimulation of proinflammatory cytokines. NOS2 plays an important role in host defense against pathogens and participates in anti-tumor processes.
Interaction
Forms a homodimer. Interacts with SLC9A3R1, GAPDH, S100A8, and S100A9.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Salim T, et al. 2016. PLoS One. 11:e0153289.
2. Ma Z, et al. 2016. Inflammation. 39:543.
3. Lee SH, et al. 2016. J. Cell. Biochem. 117:978.
4. Landes MB, et al. 2015. J. Leuko. Biol. 97:1111.
5. Karpuzoglu E, et al. 2006. Nitric. Oxide 15:177.
6. Suschek CV, et al. 2004. Curr. Mol. Med. 4:763.

Gene ID
4843 View all products for this Gene ID
UniProt
View information about Nos2 on UniProt.org
Go To Top Version: 1    Revision Date: 09/26/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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