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Purified anti-Parkin Antibody

Pricing & Availability
Clone
5C3/Parkin (See other available formats)
Regulatory Status
RUO
Other Names
PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2
Isotype
Mouse IgG1, κ
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Product Citations
publications
5C3-Parkin_Purified_Parkin_Antibody_1_050919
Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5C3-Parkin_Purified_Parkin_Antibody_1_050919
    Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5C3-Parkin_Purified_Parkin_Antibody_2_050919
    Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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865601 25 µg £73
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865602 100 µg £189
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Description

Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile Parkinson’s disease (AR-JP).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810777 (BioLegend Cat. No. 865601)
AB_2810777 (BioLegend Cat. No. 865602)

Antigen Details

Structure
Parkin is a 465 amino acid protein with an apparent molecular mass of ~ 52 kD.
Distribution

Tissue Distribution: Brain, heart, testis, and skeletal muscle.
Cellular Distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum.

Function
Parkin in involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
Interaction
Ubiquitin, PINK1
Ligand/Receptor
BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Protein Misfolding and Aggregation, Ubiquitin/Protein Degradation
Molecular Family
Enzymes and Regulators, Ligases
Antigen References
  1. Tay SP, et al. 2018. J Biol Chem. 285: 29231-29238.
  2. Pawlyk AC, et al. 2003. J Biol Chem. 278(48):48120-8.
Gene ID
5071 View all products for this Gene ID
UniProt
View information about Parkin on UniProt.org

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Go To Top Version: 1    Revision Date: 05/09/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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