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Purified anti-PARP Antibody

Pricing & Availability
Clone
5A5 (See other available formats)
Regulatory Status
RUO
Other Names
Poly (ADP-ribose) polymerase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A5_PURE_PARP_Antibody_1_WB_102317
Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using PARP (5A5) Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (#405306). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 5A5_PURE_PARP_Antibody_1_WB_102317
    Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using PARP (5A5) Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (#405306). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 5A5_Pure_PARP_Antibody_2_042314
    Total lysates (15 µg protein) from 293T and 293T/PARP knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-PARP antibody, clone 5A5 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 5A5_Pure_PARP_Antibody_3_042314
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified PARP (clone 5A5) (red) in blocking buffer overnight at 4°C and followed by anti-mouse IgG Dylight™ 594 staining for 2 hours and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C . Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 5A5_Pure_PARP_Antibody_4_032422
    IHC staining using purified anti-PARP antibody (clone 5A5) on formalin-fixed paraffin-embedded tonsil tissue. The tissue was incubated with 10 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (red, both panels) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue, left panel) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 µM
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614301 25 µg £70
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614302 100 µg £152
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Description

PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial), N-terminal 2/3 sequence of PARP
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL (1:500-1:1000 dilution). For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa.

Application References

(PubMed link indicates BioLegend citation)
  1. Bogiatzi SI, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed.
  2. Gu Y, et al. 2013. Acta Biochim Biophys Sin. PubMed
Product Citations
  1. Aggarwal M, et al. 2023. Cancers (Basel). 15: . PubMed
  2. Nakaya M, et al. 2017. J Clin Invest. 127:383. PubMed
  3. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
  4. Bogiatzi S, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed
  5. Gu Y, et al. 2013. Acta Biochim Biophys Sin. 45:904. PubMed
RRID
AB_2299318 (BioLegend Cat. No. 614301)
AB_2299318 (BioLegend Cat. No. 614302)

Antigen Details

Structure
PARP family, BRCT domain, homo- or hetero-dimer; 113 kD
Distribution

Nuclear

Function
Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription
Interaction
Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others
Modification
Ribosylation by PARP2
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370.
2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907.
3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227.
4. Noel G, et al. 2003. BMC Cell Biol. 4:7.

Regulation
Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins
Gene ID
142 View all products for this Gene ID
UniProt
View information about PARP on UniProt.org
Go To Top Version: 9    Revision Date: 03/24/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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