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Purified anti-PTEN Antibody

Pricing & Availability
Clone
4C11A11 (See other available formats)
Regulatory Status
RUO
Other Names
MMAC1, BZS, TEP1, MHAM1, GLM2, PTEN1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_4C11A11_Purified_PTEN_Antibody_WB_2_121217
Total lysates (15 µg protein) from HeLa (Human, Positive control), Jurkat (Human, Low expression negative control), Raw264.7 (Mouse) and NIH3T3 (Mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-PTEN antibody, clone 4C11A11. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1_4C11A11_Purified_PTEN_Antibody_WB_2_121217
    Total lysates (15 µg protein) from HeLa (Human, Positive control), Jurkat (Human, Low expression negative control), Raw264.7 (Mouse) and NIH3T3 (Mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-PTEN antibody, clone 4C11A11. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 2_4C11A11_Purified_PTEN_Antibody_1_WB_072216
    Total cell lysate from NIH3T3 cells (lane 1, 15 µg), PC3 cells (lane 2, 15 ug) and HeLa cells (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-PTEN antibody (clone 4C11A11) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-β-actin antibody (poly6221) was used as a loading control.
  • 3_4C11A11_Purified_PTEN_Antibody_2_IF_031913
    MCF7 cells were stained with purified anti-PTEN (4C11A11) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
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655002 100 µg £182
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Description

PTEN was discovered as a tumor suppressor with lipid and protein phosphatase activity. Heterozygous loss of PTEN in mice contribute to the development of a variety of types of cancer, suggesting that insufficient PTEN activity results in increased cancer susceptibility. PTEN negatively regulates the PI3K/AKT pathway by dephosphorylating the second messenger phosphoinositide 3,4,5-triphosphate (PIP3) produced by phosphoinositide 3-kinase (PI3K), which results in inhibition of abnormal cell proliferation. Despite the hydrolysis function of PIP3, the nuclear population of PTEN possesses greater tumor suppressive activities, regulates gene expression, differentiation, and cell cycle progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human PTEN recombinant protein (303-403aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 2.5 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The binding of PTEN antibody (4C11A11) to its target is sensitive to salt concentration. Please use the Biolegend recommended TBS/T buffer for Western blotting.

Product Citations
  1. Batth IS, et al. 2020. BMC Cancer. 20(1):715. PubMed
  2. Ren W, et al. 2019. J Exp Clin Cancer Res. 38:62. PubMed
RRID
AB_2561892 (BioLegend Cat. No. 655002)

Antigen Details

Structure
403 amino acids, predicted molecular weight of 47 kD, and observed molecular weight of 54 kD. Contains a C2 tensin-like domain responsible for lipid binding and membrane localization.
Distribution

Cytoplasm (unubiquitinated form); nucleus (mono-ubiquitinated form).

Function
PTEN functions as a tumor suppressor, modulating the PI3K-AKT-mTOR signaling pathway. It exerts phosphatase activity, dephosphorylating serine, threonine, and tyrosine phosphorylated proteins. In addition, dephosphorylates phospholipids, removing a phosphate from phosphatidylinositol-3,4,5-trisphosphate. The lipid phosphatase activity is required for its tumor suppressor function.
Interaction
Interacts with MAIG2, MAIG3, MAST1, MAST3, NEDD4, NDFIP1, NDFIP2, USP7, ROCK1, and XIAP. The unphosphorylated form interacts with AIP1, DLG1 and MAS.T2.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Carracedo A, et al. 2011. Cancer Res. 71:629.
2. Jiang BH and Liu LZ. 2009. Adv Cancer Res. 102:19.
3. Mao JH, et al. 2008. Science 321:1499.
4. Weng LP, et al. 2001. Hum. Mol. Genet. 10:599.
5. Wrighton KH. 2011. Nat Rev Cancer. 11:154.
6. Wang X, et al. 2007. Cell 128:129.

Gene ID
5728 View all products for this Gene ID
UniProt
View information about PTEN on UniProt.org
Go To Top Version: 6    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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