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Purified anti-rabbit IgG Antibody

Pricing & Availability
Clone
6B9G9 (See other available formats)
Regulatory Status
RUO
Other Names
N0034
Isotype
Mouse IgG1, κ
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Product Citations
publications
6B9G9_Purified_IgG_Antibody_1_111416
Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with purified anti-GAPDH (clone Poly6314, rabbit IgG) antibody (lane 1) and purified anti-β-actin (clone 2F1-1, mouse IgG) antibody (lane 2). After a 30 minute wash, both lanes were further probed with purified anti-rabbit IgG (clone 6B9G9) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • 6B9G9_Purified_IgG_Antibody_1_111416
    Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with purified anti-GAPDH (clone Poly6314, rabbit IgG) antibody (lane 1) and purified anti-β-actin (clone 2F1-1, mouse IgG) antibody (lane 2). After a 30 minute wash, both lanes were further probed with purified anti-rabbit IgG (clone 6B9G9) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • 6B9G9_Purified_IgG_Antibody_2_111416
    Immunofluorescence of HeLa cells without (A) or with (B-D) pericentrin Rabbit primary antibody (Cat. No. 923701). Rabbit IgG mouse antibody was used as the secondary antibody (Cat. No. 410404). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as tertiary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A-D) is 1/15. Concentrations for (A, B) is 1 µg/ml, (C) is 0.5 µg/ml, (D) is 0.2 µg/ml.
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410404 100 µg £221
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Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rabbit IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml (Dilution 1:500-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632606 (BioLegend Cat. No. 410404)

Antigen Details

Structure
IgG antibodies are large molecules of about 150 kD made of four peptide chains. It contains two identical class γ heavy chains of about 50 kD and two identical light chains of about 25 kD.
Distribution

Secreted, B cell surface.

Function
Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
Gene ID
100009097 View all products for this Gene ID
UniProt
View information about IgG on UniProt.org
Go To Top Version: 1    Revision Date: 11/13/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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