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Purified anti-SNAP-25 Antibody

Pricing & Availability
Clone
O91D3/SNAP-25 (See other available formats)
Regulatory Status
RUO
Other Names
SNAP-25, Synaptosomal-associated protein 25
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_O91D3slashSNAP25_PU_SNAP25_WB_121118
Western blot of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_O91D3slashSNAP25_PU_SNAP25_WB_121118
    Western blot of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_O91D3slashSNAP25_PU_SNAP25_IHCP_HuBrain_121118
    IHC staining of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_O91D3slashSNAP25_PU_SNAP25_IHCP_MuBrain_121118
    IHC staining of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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850301 25 µg £77
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850302 100 µg £189
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Description

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human SNAP-25 protein (NP_003072) produced in HEK293T cell.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Dentoni G, et al. 2022. J Alzheimers Dis. 90:565. PubMed
  2. Dentoni G, et al. 2022. Cells. 11:. PubMed
  3. Naia L, et al. 2021. BMC Biology. 19(1):57. PubMed
RRID
AB_2715872 (BioLegend Cat. No. 850301)
AB_2715872 (BioLegend Cat. No. 850302)

Antigen Details

Structure
SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus and cell membrane.

Function
SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction
SNAP-25 has been found to be part of the SNARE core complex containing SNAP25, VAMP2 and STX1A). SNAP-25 was also found in a complex containing SYT1, SV2B and syntaxin-1. SNAP-25 interacts with KCNB1 (via N-terminus), which reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. In developing brain and cell cultures, SNAP-25 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type
Mesenchymal Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References

1. Egbujo CN, et al. 2016. Curr. Psychiatry Rep. 18(8):77.
2. Antonucci F, et al. 2016. Front. Synaptic Neurosci. 8:7.
3. Cupertino RB, et al. 2016. J. Neural. Transm. (Vienna). 123(8):867.

Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org
Go To Top Version: 2    Revision Date: 12/11/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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