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Purified anti-STAT1 Antibody

Pricing & Availability
Clone
10C4B40 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_10C4B40_PURE_STAT1_Antibody_WB_101520.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-STAT1 antibody (clone 10C4B40) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 1_10C4B40_PURE_STAT1_Antibody_WB_101520.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-STAT1 antibody (clone 10C4B40) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 2_10C4B40_PURE_STAT1_Antibody_IP_101520.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102) or purified anti-STAT1 antibody, clone 10C4B40. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with clone 10C4B40. Lane M: Molecular weight marker.
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661001 25 µg £61
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661002 100 µg £182
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml (1:500). For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test.It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Morisaki Y, et al. 2023. Front Cell Neurosci. 1308972:17. PubMed
  2. Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed
RRID
AB_2563664 (BioLegend Cat. No. 661001)
AB_2563664 (BioLegend Cat. No. 661002)

Antigen Details

Structure
750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Stem Cells, Transcription Factors
Antigen References

1. Durbin JE, et al. 1996. Cell 8:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell 93:827.
4. Ramana CV, et al. 2000. Oncogene 19:2619.

Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10/15/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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