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Purified anti-STAT5 Antibody

Pricing & Availability
Clone
9C8B50 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 5A
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_9C8B50_Purified_STAT5_Antibody_1_122117
Total lysates (15 µg protein) from 293T and 293T/STAT5 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-STAT5 antibody, clone 9C8B50 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 1_9C8B50_Purified_STAT5_Antibody_1_122117
    Total lysates (15 µg protein) from 293T and 293T/STAT5 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-STAT5 antibody, clone 9C8B50 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 2_9C8B50_PURE_STAT5_Antibody_WB_011618
    Total lysates (15µg protein) from Jurkat (lane1), PC3 (lane 2) and Raw264.7 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-STAT5 Antibody, clone 9C8B50 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-STAT5 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 3_9C8B50_Purified_STAT5_Antibody_3_122117
    Hela cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti-STAT5 Antibody (9C8B50) and followed by DyLight™ 594 goat anti-mouse IgG (red) for 1 hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 4_9C8B50_Purified_STAT5_Antibody_4_122117
    Immunoprecipitation of STAT5 from Jurkat cell extracts. Lane 1 is 8% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-STAT5 antibody (clone 9C8B50, lane 4). Western blot was performed using anti-STAT5 antibody (clone 9C8B50).
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660202 100 µg £206
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Description

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant.  Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT5A recombinant protein (627-794 a.a.) expressed in E. coli.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10.0 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Zeng H, et al. 2018. Sci Adv. 4:eaar5701. PubMed
RRID
AB_2563469 (BioLegend Cat. No. 660202)

Antigen Details

Structure
794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Interaction
Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
Cell Type
B cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
2. Cholez E, et al. 2012. Leukemia. 26:2390.
3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
6. Xu J, et al. 2009. J. Neurosci. 29:2022.

Gene ID
6776 View all products for this Gene ID
UniProt
View information about STAT5 on UniProt.org
Go To Top Version: 7    Revision Date: 01/16/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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